doi: 10.1038/nsmb1107.
Epub 2006 Jun 18.
Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles
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- PMID: 16783376
- DOI: 10.1038/nsmb1107
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Cis-preferential LINE-1 reverse transcriptase activity in ribonucleoprotein particles
Nat Struct Mol Biol.
2006 Jul.
Abstract
LINE-1 retrotransposons (L1s) constitute approximately 17% of human DNA, and their activity continues to affect genome evolution. Retrotransposition-competent human L1s encode two proteins required for their mobility (ORF1p and ORF2p); however, biochemical activities associated with ORF2p have been difficult to detect in cells. Here, we show for the first time the colocalization of L1 RNA, ORF1p and ORF2p to a putative ribonucleoprotein retrotransposition intermediate. We further demonstrate that ORF2p preferentially uses its encoding RNA as a template for reverse transcription. Thus, our data provide the first biochemical evidence supporting the cis-preferential action of the L1 reverse transcriptase.
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