Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies
- PMID: 16761725
- DOI: 10.1385/1-59745-005-7:139
Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies
Abstract
Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods to localize these modifications within mammalian nuclei and to relate them to specific spatiotemporal patterns of DNA replication.
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