doi: 10.1128/aem.57.8.2283-2286.1991.
Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences
Affiliations
- PMID: 1662931
- PMCID: PMC183564
- DOI: 10.1128/aem.57.8.2283-2286.1991
Item in Clipboard
Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences
Appl Environ Microbiol.
1991 Aug.
Abstract
Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspension still contained some colloidal soil particles, which inhibited polymerase chain reaction amplification, a new "double" polymerase chain reaction method of analysis was adopted for amplification of Tn5-specific gene sequences. This new protocol allowed rapid detection of small numbers (1 to 10 CFU/g) of bacterial cells present in soil samples.
Similar articles
-
A simple mini-method to extract DNA directly from soil for use with polymerase chain reaction amplification.Curr Microbiol. 1993 Aug;27(2):115-8. doi: 10.1007/BF01570868. Curr Microbiol. 1993. PMID: 7763789
-
Direct recovery and molecular analysis of DNA and RNA from soil.Microb Releases. 1992 Jun;1(1):41-6. Microb Releases. 1992. PMID: 1285255
-
Monitoring genetically modified rhizobia in field soils using the polymerase chain reaction.J Appl Microbiol. 1998 Jun;84(6):1025-34. doi: 10.1046/j.1365-2672.1998.00443.x. J Appl Microbiol. 1998. PMID: 9717287
-
An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis.Curr Microbiol. 1994 Nov;29(5):301-7. doi: 10.1007/BF01577445. Curr Microbiol. 1994. PMID: 7765420
-
Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.Appl Environ Microbiol. 1992 Sep;58(9):2717-22. doi: 10.1128/aem.58.9.2717-2722.1992. Appl Environ Microbiol. 1992. PMID: 1444380 Free PMC article.
Cited by
-
Application of density gradient for the isolation of the fecal microbial stool component and the potential use thereof.Sci Rep. 2015 Nov 19;5:16807. doi: 10.1038/srep16807. Sci Rep. 2015. PMID: 26581409 Free PMC article.
-
Thymidine incorporation of bacteria sequentially extracted from soil using repeated homogenization-centrifugation.Microb Ecol. 1996 Mar;31(2):153-66. doi: 10.1007/BF00167861. Microb Ecol. 1996. PMID: 24185739
-
Extraction of bacterial RNA from soil: challenges and solutions.Microbes Environ. 2012;27(2):111-21. doi: 10.1264/jsme2.me11304. Microbes Environ. 2012. PMID: 22791042 Free PMC article. Review.
-
An improved method for extracting bacteria from soil for high molecular weight DNA recovery and BAC library construction.J Microbiol. 2010 Dec;48(6):728-33. doi: 10.1007/s12275-010-0139-1. Epub 2011 Jan 9. J Microbiol. 2010. PMID: 21221926
-
Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR.Appl Environ Microbiol. 2001 Feb;67(2):972-6. doi: 10.1128/AEM.67.2.972-976.2001. Appl Environ Microbiol. 2001. PMID: 11157271 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
