Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
- PMID: 16628208
- DOI: 10.1038/nmeth874
Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
Abstract
Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.
Comment in
-
Imaging activity in brain cells: deconvolution clears the haze.Nat Methods. 2006 May;3(5):344-6. doi: 10.1038/nmeth0506-344. Nat Methods. 2006. PMID: 16628203 No abstract available.
Similar articles
-
Optimized temporally deconvolved Ca²⁺ imaging allows identification of spatiotemporal activity patterns of CA1 hippocampal ensembles.Neuroimage. 2014 Jul 1;94:239-249. doi: 10.1016/j.neuroimage.2014.03.030. Epub 2014 Mar 17. Neuroimage. 2014. PMID: 24650598
-
Detecting action potentials in neuronal populations with calcium imaging.Methods. 1999 Jun;18(2):215-21. doi: 10.1006/meth.1999.0774. Methods. 1999. PMID: 10356353
-
Pharmacological analysis of ionotropic glutamate receptor function in neuronal circuits of the zebrafish olfactory bulb.PLoS One. 2008 Jan 9;3(1):e1416. doi: 10.1371/journal.pone.0001416. PLoS One. 2008. PMID: 18183297 Free PMC article.
-
Reporting neural activity with genetically encoded calcium indicators.Brain Cell Biol. 2008 Aug;36(1-4):69-86. doi: 10.1007/s11068-008-9029-4. Epub 2008 Oct 22. Brain Cell Biol. 2008. PMID: 18941901 Free PMC article. Review.
-
Optical monitoring of brain function in vivo: from neurons to networks.Pflugers Arch. 2006 Dec;453(3):385-96. doi: 10.1007/s00424-006-0150-x. Epub 2006 Oct 18. Pflugers Arch. 2006. PMID: 17047983 Review.
Cited by
-
Monitoring activity in neural circuits with genetically encoded indicators.Front Mol Neurosci. 2014 Dec 5;7:97. doi: 10.3389/fnmol.2014.00097. eCollection 2014. Front Mol Neurosci. 2014. PMID: 25538558 Free PMC article. Review.
-
Analyzing the structure and function of neuronal circuits in zebrafish.Front Neural Circuits. 2013 Apr 23;7:71. doi: 10.3389/fncir.2013.00071. eCollection 2013. Front Neural Circuits. 2013. PMID: 23630467 Free PMC article. Review.
-
Role of intraglomerular circuits in shaping temporally structured responses to naturalistic inhalation-driven sensory input to the olfactory bulb.J Neurophysiol. 2015 May 1;113(9):3112-29. doi: 10.1152/jn.00394.2014. Epub 2015 Feb 25. J Neurophysiol. 2015. PMID: 25717156 Free PMC article.
-
Probing the coding logic of thermosensation using spinal cord calcium imaging.Exp Neurol. 2019 Aug;318:42-49. doi: 10.1016/j.expneurol.2019.04.009. Epub 2019 Apr 20. Exp Neurol. 2019. PMID: 31014574 Free PMC article. Review.
-
The network and the synapse: 100 years after Cajal.HFSP J. 2008 Feb;2(1):12-6. doi: 10.2976/1.2835214. Epub 2008 Jan 30. HFSP J. 2008. PMID: 19404449 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
