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. 1991 Dec 13;254(5038):1647-51.
doi: 10.1126/science.1661029.

Cell-free, de novo synthesis of poliovirus

A Molla  1 , A V Paul, E Wimmer
Affiliations

Cell-free, de novo synthesis of poliovirus

A Molla et al. Science. .

Abstract

Cell-free translation of poliovirus RNA in an extract of uninfected human (HeLa) cells yielded viral proteins through proteolysis of the polyprotein. In the extract, newly synthesized proteins catalyzed poliovirus-specific RNA synthesis, and formed infectious poliovirus de novo. Newly formed virions were neutralized by type-specific antiserum, and infection of human cells with them was prevented by poliovirus receptor-specific antibodies. Poliovirus synthesis was increased nearly 70-fold when nucleoside triphosphates were added, but it was abolished in the presence of inhibitors of translation or viral genome replication. The ability to conduct cell-free synthesis of poliovirus will aid in the study of picornavirus proliferation and in the search for the control of picornaviral disease.

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