Differential regulation of three members of the ATF/CREB family of DNA-binding proteins
- PMID: 1658708
Differential regulation of three members of the ATF/CREB family of DNA-binding proteins
Abstract
The ATF/CRE binding site can mediate transcriptional activation by cAMP, the adenovirus E1A protein and the human T-cell leukaemia virus 1 (HTLV1) tax protein. A large number of different proteins bind specifically to this element either as homodimers or as heterodimers. Using GAL4-ATF/CREB fusions, we have investigated the regulatory functions of three members of this family. CREB1 (CREB) is strongly activated by cAMP and weakly activated by the E1A protein. In contrast, CREB2 (CRE-BP1, ATF2) is strongly activated by E1A but is insensitive to cAMP stimulation. ATF1 is weakly activated by cAMP but is not activated by E1A. All three proteins are insensitive to activation by the HTLV1 tax protein. The N-terminal region of CREB2, from amino acid residues 19 to 112, is both necessary and sufficient for E1A activation. This region contains a putative C2H2 metal-binding finger, and single amino acid substitutions of the cysteine residues severely decreased CREB2 activity. In contrast, mutations affecting a potential protein kinase A and casein kinase II phosphorylation site within this region had little effect.
Similar articles
-
Cyclic AMP response element-binding protein, CRE-BP1, mediates the E1A-induced but not the Tax-induced trans-activation.Oncogene. 1991 Apr;6(4):627-32. Oncogene. 1991. PMID: 1827668
-
ATF1 and CREB trans-activate a cell cycle regulated histone H4 gene at a distal nuclear matrix associated promoter element.Biochemistry. 1997 Nov 25;36(47):14447-55. doi: 10.1021/bi971781s. Biochemistry. 1997. PMID: 9398163
-
Late induction of CREB/ATF binding and a concomitant increase in cAMP levels in T and B lymphocytes stimulated via the antigen receptor.J Immunol. 1996 Jun 15;156(12):4582-93. J Immunol. 1996. PMID: 8648100
-
The CREB/ATF family of transcription factors: modulation by reversible phosphorylation.Anticancer Res. 1994 Sep-Oct;14(5A):1801-5. Anticancer Res. 1994. PMID: 7847813 Review.
-
Hormonal control of gene expression: multiplicity and versatility of cyclic adenosine 3',5'-monophosphate-responsive nuclear regulators.Mol Endocrinol. 1993 Feb;7(2):145-53. doi: 10.1210/mend.7.2.8385737. Mol Endocrinol. 1993. PMID: 8385737 Review. No abstract available.
Cited by
-
The PHD type zinc finger is an integral part of the CBP acetyltransferase domain.Mol Cell Biol. 2002 Apr;22(7):1961-70. doi: 10.1128/MCB.22.7.1961-1970.2002. Mol Cell Biol. 2002. PMID: 11884585 Free PMC article.
-
Promoter activity of the proliferating-cell nuclear antigen gene is associated with inducible CRE-binding proteins in interleukin 2-stimulated T lymphocytes.Mol Cell Biol. 1994 Jun;14(6):4233-43. doi: 10.1128/mcb.14.6.4233-4243.1994. Mol Cell Biol. 1994. PMID: 7910946 Free PMC article.
-
An ATF/CRE element mediates both EBNA2-dependent and EBNA2-independent activation of the Epstein-Barr virus LMP1 gene promoter.J Virol. 1998 Feb;72(2):1365-76. doi: 10.1128/JVI.72.2.1365-1376.1998. J Virol. 1998. PMID: 9445037 Free PMC article.
-
ATF3 and stress responses.Gene Expr. 1999;7(4-6):321-35. Gene Expr. 1999. PMID: 10440233 Free PMC article. Review.
-
TIP49b, a regulator of activating transcription factor 2 response to stress and DNA damage.Mol Cell Biol. 2001 Dec;21(24):8398-413. doi: 10.1128/MCB.21.24.8398-8413.2001. Mol Cell Biol. 2001. PMID: 11713276 Free PMC article.