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. 1991 Sep;10(9):2553-8.
doi: 10.1002/j.1460-2075.1991.tb07795.x.

Vaccinia virus capping enzyme is a transcription initiation factor

J C Vos  1 , M Sasker, H G Stunnenberg
Affiliations

Vaccinia virus capping enzyme is a transcription initiation factor

J C Vos et al. EMBO J. 1991 Sep.

Abstract

It has previously been demonstrated that vaccinia virus capping enzyme is involved both in the formation of a 5' cap structure and in termination of early transcription. Here we show that capping enzyme has an additional activity which is required for transcription of intermediate genes. VITF-A and VITF-B have been defined as two activities which together with RNA polymerase are necessary and sufficient to transcribe intermediate genes in vitro. VITF-A and the viral capping enzyme are shown to copurify to near homogeneity. Direct evidence that capping enzyme is VITF-A was obtained by complementation of a reconstituted transcription system with viral capping enzyme expressed in Escherichia coli. Although capping enzyme is a cofactor in early transcription termination, intermediate transcription is not terminated in response to the early termination signal. Capping enzyme is shown to form a complex with RNA polymerase in the absence of VITF-B. This appears to be a prerequisite for the formation of a stable initiation complex.

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