Identification of mouse orthologue of endogenous secretory receptor for advanced glycation end-products: structure, function and expression
- PMID: 16503878
- PMCID: PMC1450004
- DOI: 10.1042/BJ20051573
Identification of mouse orthologue of endogenous secretory receptor for advanced glycation end-products: structure, function and expression
Abstract
The cell-surface RAGE [receptor for AGE (advanced glycation end-products)] is associated with the development of diabetic vascular complications, neurodegenerative disorders and inflammation. Recently, we isolated a human RAGE splice variant, which can work as a decoy receptor for RAGE ligands, and named it esRAGE (endogenous secretory RAGE). In the present study, we have isolated the murine equivalent of esRAGE from brain polysomal poly(A)+ (polyadenylated) RNA by RT (reverse transcription)-PCR cloning. The mRNA was generated by alternative splicing, and it encoded a 334-amino-acid protein with a signal sequence, but lacking the transmembrane domain. A transfection experiment revealed that the mRNA was actually translated as deduced to yield the secretory protein working as a decoy in AGE-induced NF-kappaB (nuclear factor kappaB) activation. RT-PCR and immunoblotting detected esRAGE mRNA and protein in the brain, lung, kidney and small intestine of wild-type mice, but not of RAGE-null mice. The esRAGE expression was increased in the kidney of diabetic wild-type mice. The present study has thus provided an animal orthologue of esRAGE for clarification of its roles in health and disease.
Figures





Similar articles
-
Novel splice variants of the receptor for advanced glycation end-products expressed in human vascular endothelial cells and pericytes, and their putative roles in diabetes-induced vascular injury.Biochem J. 2003 Mar 15;370(Pt 3):1097-109. doi: 10.1042/BJ20021371. Biochem J. 2003. PMID: 12495433 Free PMC article.
-
Receptor for advanced glycation end products is involved in impaired angiogenic response in diabetes.Diabetes. 2006 Aug;55(8):2245-55. doi: 10.2337/db05-1375. Diabetes. 2006. PMID: 16873687
-
Endogenous secretory receptor for advanced glycation end-products and cardiovascular disease in end-stage renal disease.J Ren Nutr. 2008 Jan;18(1):76-82. doi: 10.1053/j.jrn.2007.10.016. J Ren Nutr. 2008. PMID: 18089449 Review.
-
Receptor for advanced glycation end products (RAGE) mediates neuronal differentiation and neurite outgrowth.J Neurosci Res. 2008 May 1;86(6):1254-66. doi: 10.1002/jnr.21578. J Neurosci Res. 2008. PMID: 18058943
-
RAGE axis: Animal models and novel insights into the vascular complications of diabetes.Arterioscler Thromb Vasc Biol. 2004 Aug;24(8):1342-9. doi: 10.1161/01.ATV.0000133191.71196.90. Epub 2004 May 20. Arterioscler Thromb Vasc Biol. 2004. PMID: 15155381 Review.
Cited by
-
Oxytocin Dynamics in the Body and Brain Regulated by the Receptor for Advanced Glycation End-Products, CD38, CD157, and Nicotinamide Riboside.Front Neurosci. 2022 Jul 7;16:858070. doi: 10.3389/fnins.2022.858070. eCollection 2022. Front Neurosci. 2022. PMID: 35873827 Free PMC article. Review.
-
Complex tissue-specific patterns and distribution of multiple RAGE splice variants in different mammals.Genome Biol Evol. 2013;5(12):2420-35. doi: 10.1093/gbe/evt188. Genome Biol Evol. 2013. PMID: 24273313 Free PMC article.
-
RAGE does not contribute to renal injury and damage upon ischemia/reperfusion-induced injury.J Innate Immun. 2012;4(1):80-5. doi: 10.1159/000334251. Epub 2011 Nov 4. J Innate Immun. 2012. PMID: 22067944 Free PMC article.
-
High BAL sRAGE is Associated with Low Serum Eosinophils and IgE in Children with Asthma.Children (Basel). 2020 Aug 24;7(9):110. doi: 10.3390/children7090110. Children (Basel). 2020. PMID: 32846877 Free PMC article.
-
Alternative splicing of the RAGE cytoplasmic domain regulates cell signaling and function.PLoS One. 2013 Nov 8;8(11):e78267. doi: 10.1371/journal.pone.0078267. eCollection 2013. PLoS One. 2013. PMID: 24260107 Free PMC article.
References
-
- Schmidt A. M., Stern D. M. RAGE: a new target for the prevention and treatment of the vascular and inflammatory complications of diabetes. Trends Endocrinol. Metab. 2000;11:368–375. - PubMed
-
- Neeper M., Schmidt A. M., Brett J., Yan S. D., Wang F., Pan Y. C. E., Elliston K., Stern D., Shaw A. Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins. J. Biol. Chem. 1992;267:14998–15004. - PubMed
-
- Hofmann M. A., Drury S., Fu C., Qu W., Taguchi A., Lu Y., Avil C., Kambham N., Bierhaus A., Nawroth P., et al. RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 1999;97:889–901. - PubMed
-
- Huttunen H. J., Fages C., Rauvala H. Receptor for advanced glycation end products (RAGE)-mediated neurite outgrowth and activation of NF-κB required the cytoplasmic domain of the receptor but different downstream signaling pathway. J. Biol. Chem. 1999;274:19919–19924. - PubMed
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Molecular Biology Databases