Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2006 Mar;14(3):141-7.
doi: 10.1016/j.tim.2006.01.006. Epub 2006 Feb 7.

Phage display in the study of infectious diseases

Lisa M Mullen  1 , Sean P Nair, John M Ward, Andrew N Rycroft, Brian Henderson
Affiliations
Review

Phage display in the study of infectious diseases

Lisa M Mullen et al. Trends Microbiol. 2006 Mar.

Abstract

Microbial infections are dependent on the panoply of interactions between pathogen and host and identifying the molecular basis of such interactions is necessary to understand and control infection. Phage display is a simple functional genomic methodology for screening and identifying protein-ligand interactions and is widely used in epitope mapping, antibody engineering and screening for receptor agonists or antagonists. Phage display is also used widely in various forms, including the use of fragment libraries of whole microbial genomes, to identify peptide-ligand and protein-ligand interactions that are of importance in infection. In particular, this technique has proved successful in identifying microbial adhesins that are vital for colonization.

PubMed Disclaimer

Figures

Figure 1
Figure 1
A cycle of affinity selection of filamentous phage display libraries to identify phage that display fusion proteins (pIII fusions) that bind to the chosen ligand or ligands.
Figure 2
Figure 2
Dimensions and architecture of filamentous bacteriophage fd. The copy number of each protein is shown in brackets.
Figure 3
Figure 3
Life cycle of filamentous bacteriophage in Escherichia coli. The drawing is schematic and not to scale. (1) Phage bind to the E. coli cell through the pIII coat protein. The single-stranded viral genome (+ strand, single circle) is injected into the cell and a complementary strand (− strand) is synthesized to form a double-stranded phage genome (double circle). (2) Subsequently, all ten phage-encoded proteins are produced by host-mediated protein synthesis, including coat proteins (pIII, pVI, pVII, pVIII and pIX), proteins for replication (pII, pV and pX) and proteins involved in assembly and export (pI and pIV). (3) The phage genome is replicated using the (+)-strand as a primer and the (−)-strand as a template. (4) Virions are assembled and exported across the bacterial membranes.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Smith G.P. Filamentous fusion phage – novel expression vectors that display cloned antigens on the virion surface. Science. 1985;228:1315–1317. - PubMed
    1. Russel M. Introduction to phage display and phage biology. In: Clackson T., Lowman H., editors. Phage Display: A Practical Approach. Oxford University Press; 2004. pp. 1–26.
    1. Azzazy H.M.E., Highsmith J.W.E. Phage display technology: clinical applications and recent innovations. Clin. Biochem. 2002;35:425–445. - PubMed
    1. Castagnoli L. Alternative bacteriophage display systems. Comb. Chem. High Throughput Screen. 2001;4:121–133. - PubMed
    1. Lindqvist B.H., Naderi S. Peptide presentation by bacteriophage P4. FEMS Microbiol. Rev. 1995;17:33–39. - PubMed