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. 2006 Feb;3(2):95-7.
doi: 10.1038/nmeth850.

BEAMing up for detection and quantification of rare sequence variants

Meng Li  1 , Frank Diehl, Devin Dressman, Bert Vogelstein, Kenneth W Kinzler
Affiliations

BEAMing up for detection and quantification of rare sequence variants

Meng Li et al. Nat Methods. 2006 Feb.

Abstract

BEAMing allows the one-to-one conversion of a population of DNA fragments into a population of beads. We used rolling circle amplification to increase the number of copies bound to such beads by more than 100-fold. This allowed enumeration of mutant and wild-type sequences even when they were present at ratios less than 1:10,000 and was sensitive enough to directly quantify the error rate of DNA polymerases used for PCR.

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