Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Sep-Oct;88(5):1279-91.

Determination of beta-carotene in supplements and raw materials by reversed-phase high pressure liquid chromatography: collaborative study

John Szpylka  1 , Jonathan W DeVries
Affiliations

Determination of beta-carotene in supplements and raw materials by reversed-phase high pressure liquid chromatography: collaborative study

John Szpylka et al. J AOAC Int. 2005 Sep-Oct.

Abstract

Twelve laboratories representing 4 countries participated in an interlaboratory study conducted to determine all-trans-veta-carotene and total beta-carotene in dietary supplements and raw materials. Thirteen samples were sent as blind duplicates to the collaborators. Results obtained from 11 laboratories are reported. For products composed as softgels and tablets that were analyzed for total beta-carotene, the reproducibility relative standard deviation (RSDR) ranged from 3.35 to 23.09% and the HorRat values ranged from 1.06 to 3.72. For these products analyzed for trans beta-carotene, the reproducibility relative standard deviation (RSDR) ranged from 4.28 to 22.76% and the HorRat values ranged from 0.92 to 3.37. The RSDr and HorRat values in the analysis of a beadlet raw material were substantial and it is believed that the variability within the material itself introduced significant variation in subsampling. The method uses high pressure liquid chromatography (LC) in the reversed-phase mode with visible light absorbance for detection and quantitation. If high levels of alpha-carotenes are present, a second LC system is used for additional separation and quantitation of the carotene species. It is recommended that the method be adopted as an AOAC Official Method.

PubMed Disclaimer

Figures

Figure 2005.07A
Figure 2005.07A
Chromatograms using LC System A. (A) Control sample; (B) multivitamin tablet sample 108.
Figure 2005.07B
Figure 2005.07B
Chromatograms using LC System B. (A) Control sample; (B) softgel sample set 2, No. 2.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

Publication types

MeSH terms

LinkOut - more resources