Detection of immune responses against urinary bladder cancer in sentinel lymph nodes
- PMID: 16321468
- DOI: 10.1016/j.eururo.2005.09.010
Detection of immune responses against urinary bladder cancer in sentinel lymph nodes
Abstract
Objective: The lymphatic drainage from a tumour is received in the sentinel node where the immune system encounters tumour derived antigens. We investigated anti-tumoural lymphocyte function in sentinel nodes from patients with urinary bladder cancer.
Methods: In 14 patients undergoing cystectomy due to bladder cancer, radioactive tracer and blue dye were used to identify the sentinel node. Cell suspensions from the tumour, sentinel- and non-sentinel nodes and peripheral blood were analyzed by flow cytometry with antibodies against lymphocyte surface antigens and against the tumour cell marker cytokeratin-20. Reactivity against autologous tumour extract and the mitogen Concanavalin A was tested in proliferation assays with 3H-Thymidine incorporation. Lymphocytes were put in long-term culture with IL-2 and autologous tumour extract.
Results: Sentinel nodes were detected in 12 of the 14 patients. Antigen dependent proliferation in response to autologous tumour extract was detected in 6 patients, in 5 cases in sentinel nodes, in the remaining case in a non-sentinel node. Proliferation against Concanavalin A was vigorous in lymph nodes from all patients, whereas tumour infiltrating lymphocytes were unresponsive. Lymphocytes from sentinel nodes could be expanded in vitro.
Conclusion: Tumour reactive lymphocytes are present in sentinel nodes draining human bladder cancers. These cells display immunologic function upon restimulation in vitro, and provide a promising source for expansion and subsequent adoptive T cell immunotherapy.
Similar articles
-
Lymphatic mapping and detection of sentinel nodes in patients with bladder cancer.J Urol. 2001 Sep;166(3):812-5. J Urol. 2001. PMID: 11490224
-
Feasibility of T-cell-based adoptive immunotherapy in the first 12 patients with advanced urothelial urinary bladder cancer. Preliminary data on a new immunologic treatment based on the sentinel node concept.Eur Urol. 2010 Jul;58(1):105-11. doi: 10.1016/j.eururo.2009.09.026. Epub 2009 Sep 11. Eur Urol. 2010. PMID: 19766386 Clinical Trial.
-
Maturation of dendritic cells and T-cell responses in sentinel lymph nodes from patients with breast carcinoma.Cancer. 2006 Mar 15;106(6):1227-36. doi: 10.1002/cncr.21729. Cancer. 2006. PMID: 16475148
-
[Sentinel lymph-node biopsy for breast cancer. Analysis of 235 cases and review of the literature].Chir Ital. 2006 Sep-Oct;58(5):583-95. Chir Ital. 2006. PMID: 17069187 Review. Italian.
-
Recent advances in the immunobiology of human bladder cancer.Clin Bull. 1975;5(4):149-53. Clin Bull. 1975. PMID: 776446 Review. No abstract available.
Cited by
-
Sentinel node lymphocytes: tumour reactive lymphocytes identified intraoperatively for the use in immunotherapy of colon cancer.Br J Cancer. 2006 May 22;94(10):1478-84. doi: 10.1038/sj.bjc.6603126. Br J Cancer. 2006. PMID: 16641897 Free PMC article.
-
Methods of Sentinel Lymph Node Detection and Management in Urinary Bladder Cancer-A Narrative Review.Curr Oncol. 2022 Feb 23;29(3):1335-1348. doi: 10.3390/curroncol29030114. Curr Oncol. 2022. PMID: 35323314 Free PMC article. Review.
-
Molecular Targeted Fluorescence-Guided Intraoperative Imaging of Bladder Cancer Nodal Drainage Using Indocyanine Green During Radical and Partial Cystectomy.Curr Urol Rep. 2016 Oct;17(10):74. doi: 10.1007/s11934-016-0633-z. Curr Urol Rep. 2016. PMID: 27539291 Review.
-
Fewer tumour draining sentinel nodes in patients with progressing muscle invasive bladder cancer, after neoadjuvant chemotherapy and radical cystectomy.World J Urol. 2020 Sep;38(9):2207-2213. doi: 10.1007/s00345-019-03025-w. Epub 2019 Nov 23. World J Urol. 2020. PMID: 31760442 Free PMC article.
-
Immunotherapy for urothelial carcinoma: current status and perspectives.Cancers (Basel). 2011 Jul 29;3(3):3055-72. doi: 10.3390/cancers3033055. Cancers (Basel). 2011. PMID: 24212945 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
