doi: 10.1016/S0076-6879(05)96011-4.
Detection and characterization of protein nitrosothiols
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- PMID: 16291226
- DOI: 10.1016/S0076-6879(05)96011-4
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Detection and characterization of protein nitrosothiols
Methods Enzymol.
2005.
Abstract
Protein S-nitrosylation is a post-translational modification of cysteine residues elicited by nitric oxide (NO). Detection and quantification of protein nitrosothiols remains a challenge because of the lability of the nitrosothiol moiety. Here, we describe approaches for labeling S-nitrosylated proteins with affinity and radioactive tags to facilitate their detection, purification, and identification.
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