Overlapping and distinct functions for a Caenorhabditis elegans SIR2 and DAF-16/FOXO
- PMID: 16280150
- DOI: 10.1016/j.mad.2005.09.005
Overlapping and distinct functions for a Caenorhabditis elegans SIR2 and DAF-16/FOXO
Abstract
The conserved SIR2 protein regulates life span in both yeast and worms: in both organisms overexpression of SIR2 can extend life span and in Caenorhabditis elegans this life span extension is dependent on the forkhead transcription factor, DAF-16. Here, we have done extensive genetic analysis with sir-2.1(ok434), a null mutant of C. elegans sir-2.1, the closest homolog to yeast Sir2p and human SIRT1 to further elucidate its function in life span regulation. sir-2.1(ok434) mutants show a slight decrease in life span as well as sensitivity to various stresses. Our genetic analysis suggests that sir-2.1 is required for life span extension by caloric restriction, independent of the insulin/IGF-1 signaling pathway. Importantly, analysis with unc-13 mutants indicates that sir-2.1 and daf-16 have overlapping and distinct roles in life span regulation. Our expression analysis shows that sir-2.1 has overlapping and distinct expression pattern compared with daf-16, consistent with the results from our genetic data. Our data defines a central role for C. elegans SIR2 in regulation of life span by caloric restriction and demonstrates that sir-2.1 and daf-16 have both overlapping and distinct functions in regulation of C. elegans life span.
Similar articles
-
Nicotinamide adenine dinucleotide extends the lifespan of Caenorhabditis elegans mediated by sir-2.1 and daf-16.Biogerontology. 2010 Feb;11(1):31-43. doi: 10.1007/s10522-009-9225-3. Epub 2009 Apr 16. Biogerontology. 2010. PMID: 19370397
-
Germ-cell loss extends C. elegans life span through regulation of DAF-16 by kri-1 and lipophilic-hormone signaling.Cell. 2006 Mar 10;124(5):1055-68. doi: 10.1016/j.cell.2006.01.039. Cell. 2006. PMID: 16530050
-
SMK-1, an essential regulator of DAF-16-mediated longevity.Cell. 2006 Mar 10;124(5):1039-53. doi: 10.1016/j.cell.2005.12.042. Cell. 2006. PMID: 16530049
-
Recent aging research in Caenorhabditis elegans.Exp Gerontol. 2006 Jun;41(6):557-63. doi: 10.1016/j.exger.2006.02.008. Epub 2006 Apr 3. Exp Gerontol. 2006. PMID: 16584861 Review.
-
Worming pathways to and from DAF-16/FOXO.Exp Gerontol. 2006 Oct;41(10):928-34. doi: 10.1016/j.exger.2006.05.020. Epub 2006 Jul 12. Exp Gerontol. 2006. PMID: 16839734 Review.
Cited by
-
The Lifespan-Promoting Effect of Otophylloside B in Caenorhabditis elegans.Nat Prod Bioprospect. 2015 Aug;5(4):177-183. doi: 10.1007/s13659-015-0064-4. Epub 2015 Jun 26. Nat Prod Bioprospect. 2015. PMID: 26112394 Free PMC article.
-
Calorie restriction: what recent results suggest for the future of ageing research.Eur J Clin Invest. 2010 May;40(5):440-50. doi: 10.1111/j.1365-2362.2010.02276.x. Eur J Clin Invest. 2010. PMID: 20534066 Free PMC article. Review.
-
SIRT2 regulates adipocyte differentiation through FoxO1 acetylation/deacetylation.Cell Metab. 2007 Aug;6(2):105-14. doi: 10.1016/j.cmet.2007.07.003. Cell Metab. 2007. PMID: 17681146 Free PMC article.
-
AsSIRTing the DNA damage response.Trends Cell Biol. 2008 Feb;18(2):77-83. doi: 10.1016/j.tcb.2007.11.007. Epub 2008 Jan 22. Trends Cell Biol. 2008. PMID: 18215521 Free PMC article. Review.
-
C. elegans dauer formation and the molecular basis of plasticity.Genes Dev. 2008 Aug 15;22(16):2149-65. doi: 10.1101/gad.1701508. Genes Dev. 2008. PMID: 18708575 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
