Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation
- PMID: 16222246
- DOI: 10.1038/nature04219
Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation
Abstract
Tri-methylation of histone H3 lysine 9 is important for recruiting heterochromatin protein 1 (HP1) to discrete regions of the genome, thereby regulating gene expression, chromatin packaging and heterochromatin formation. Here we show that HP1alpha, -beta, and -gamma are released from chromatin during the M phase of the cell cycle, even though tri-methylation levels of histone H3 lysine 9 remain unchanged. However, the additional, transient modification of histone H3 by phosphorylation of serine 10 next to the more stable methyl-lysine 9 mark is sufficient to eject HP1 proteins from their binding sites. Inhibition or depletion of the mitotic kinase Aurora B, which phosphorylates serine 10 on histone H3, causes retention of HP1 proteins on mitotic chromosomes, suggesting that H3 serine 10 phosphorylation is necessary for the dissociation of HP1 from chromatin in M phase. These findings establish a regulatory mechanism of protein-protein interactions, through a combinatorial readout of two adjacent post-translational modifications: a stable methylation and a dynamic phosphorylation mark.
Comment in
-
Molecular biology: antagonizing the neighbours.Nature. 2005 Dec 22;438(7071):1090-1. doi: 10.1038/4381090a. Nature. 2005. PMID: 16371991 No abstract available.
Similar articles
-
Histone H3 serine 10 phosphorylation by Aurora B causes HP1 dissociation from heterochromatin.Nature. 2005 Dec 22;438(7071):1176-80. doi: 10.1038/nature04254. Epub 2005 Oct 12. Nature. 2005. PMID: 16222244
-
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.Mol Biol Cell. 2006 Jul;17(7):3232-41. doi: 10.1091/mbc.e05-09-0906. Epub 2006 May 10. Mol Biol Cell. 2006. PMID: 16687578 Free PMC article.
-
Dynamic regulation of effector protein binding to histone modifications: the biology of HP1 switching.Cell Cycle. 2006 Dec;5(24):2842-51. doi: 10.4161/cc.5.24.3540. Epub 2006 Dec 15. Cell Cycle. 2006. PMID: 17172865
-
How HP1 Post-Translational Modifications Regulate Heterochromatin Formation and Maintenance.Cells. 2020 Jun 12;9(6):1460. doi: 10.3390/cells9061460. Cells. 2020. PMID: 32545538 Free PMC article. Review.
-
Chromosomal passengers: the four-dimensional regulation of mitotic events.Chromosoma. 2004 Nov;113(5):211-22. doi: 10.1007/s00412-004-0307-3. Epub 2004 Sep 4. Chromosoma. 2004. PMID: 15351889 Review.
Cited by
-
Characterization of histone post-translational modifications during virus infection using mass spectrometry-based proteomics.Methods. 2015 Nov 15;90:8-20. doi: 10.1016/j.ymeth.2015.06.008. Epub 2015 Jun 17. Methods. 2015. PMID: 26093074 Free PMC article.
-
Deciphering post-translational modification codes.FEBS Lett. 2013 Apr 17;587(8):1247-57. doi: 10.1016/j.febslet.2013.01.047. Epub 2013 Feb 10. FEBS Lett. 2013. PMID: 23402885 Free PMC article. Review.
-
Crosstalk between chromatin state and DNA damage response in cellular senescence and cancer.Nat Rev Cancer. 2012 Oct;12(10):709-20. doi: 10.1038/nrc3344. Epub 2012 Sep 6. Nat Rev Cancer. 2012. PMID: 22952011
-
Orchestrating chromosome conformation capture analysis with Bioconductor.Nat Commun. 2024 Feb 5;15(1):1072. doi: 10.1038/s41467-024-44761-x. Nat Commun. 2024. PMID: 38316789 Free PMC article.
-
Histone H3R2 symmetric dimethylation and histone H3K4 trimethylation are tightly correlated in eukaryotic genomes.Cell Rep. 2012 Feb 23;1(2):83-90. doi: 10.1016/j.celrep.2011.12.008. Cell Rep. 2012. PMID: 22720264 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
