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. 2005 Sep 29;437(7059):693-8.
doi: 10.1038/nature04028.

GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin

Miyako Ueguchi-Tanaka  1 , Motoyuki Ashikari, Masatoshi Nakajima, Hironori Itoh, Etsuko Katoh, Masatomo Kobayashi, Teh-yuan Chow, Yue-ie C Hsing, Hidemi Kitano, Isomaro Yamaguchi, Makoto Matsuoka
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GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin

Miyako Ueguchi-Tanaka et al. Nature. .

Abstract

Gibberellins (GAs) are phytohormones that are essential for many developmental processes in plants. It has been postulated that plants have both membrane-bound and soluble GA receptors; however, no GA receptors have yet been identified. Here we report the isolation and characterization of a new GA-insensitive dwarf mutant of rice, gid1. The GID1 gene encodes an unknown protein with similarity to the hormone-sensitive lipases, and we observed preferential localization of a GID1-green fluorescent protein (GFP) signal in nuclei. Recombinant glutathione S-transferase (GST)-GID1 had a high affinity only for biologically active GAs, whereas mutated GST-GID1 corresponding to three gid1 alleles had no GA-binding affinity. The dissociation constant for GA4 was estimated to be around 10(-7) M, enough to account for the GA dependency of shoot elongation. Moreover, GID1 bound to SLR1, a rice DELLA protein, in a GA-dependent manner in yeast cells. GID1 overexpression resulted in a GA-hypersensitive phenotype. Together, our results indicate that GID1 is a soluble receptor mediating GA signalling in rice.

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