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Comparative Study
. 2005 Sep 22;272(1575):1971-7.
doi: 10.1098/rspb.2005.3178.

Prenatal androgen exposure modulates cellular and humoral immune function of black-headed gull chicks

Wendt Müller  1 , Ton G G Groothuis, Alice Kasprzik, Cor Dijkstra, Rauno V Alatalo, Heli Siitari
Affiliations
Comparative Study

Prenatal androgen exposure modulates cellular and humoral immune function of black-headed gull chicks

Wendt Müller et al. Proc Biol Sci. .

Abstract

Avian eggs contain considerable amounts of maternal yolk androgens, which have been shown to beneficially influence the physiology and behaviour of the chick. As androgens may suppress immune functions, they may also entail costs for the chick. This is particularly relevant for colonial species, such as the black-headed gull (Larus ridibundus), in which the aggregation of large numbers of birds during the breeding season enhances the risk of infectious diseases for the hatching chick. To test the effect of maternal yolk androgens on the chick's immune function, we experimentally manipulated, in a field study, yolk androgen levels within the physiological range by in ovo injection of either androgens (testosterone and androstenedione) or sesame oil (control) into freshly laid eggs. We determined cell-mediated immunity (CMI) and humoral immunity of the chicks at the beginning of the nestling period to evaluate early modulatory effects of yolk androgens on immune function. Embryonic exposure to elevated levels of androgens negatively affected both CMI and humoral immunity in nestling gull chicks. Consequently, maternal yolk androgens not only entail benefits of enhanced competitiveness and growth as previously shown, but also costs in terms of immunosuppression. The outcome of embryonic yolk androgen exposure thus likely depends on the post-hatching circumstances for the developing offspring such as parasite exposure and degree of sibling competition.

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Figures

Figure 1
Figure 1
T-cell-mediated immunity (CMI, mean±s.e.) in relation to in ovo androgen or control treatment at day 1 (n=43 nests).
Figure 2
Figure 2
Change in plasma immunoglobulins (U ml−1) in relation to in ovo androgen treatment within 48 h after experimental challenge (n=23 nests), (a) PBS (control-challenge; n=11 nests) (b) lipopolysaccharides (LPS from E. coli) challenge (n=12 nests). Chicks were 7(±2) days old at the day of injection. Initial plasma immunoglobulin concentrations were 35 415 U ml−1 (control-chicks) and 41 066 U ml−1 (androgen-chicks), respectively.
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