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. 2005 Sep-Oct;7(5):648-56.
doi: 10.1021/cc0500607.

Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library

Hongbo Hu  1 , Li Li, Richard Y Kao, Binbin Kou, Zhanguo Wang, Liang Zhang, Huiyuan Zhang, Zhiyong Hao, Wayne H Tsui, Anping Ni, Lianxian Cui, Baoxing Fan, Feng Guo, Shuan Rao, Chengyu Jiang, Qian Li, Manji Sun, Wei He, Gang Liu
Affiliations

Screening and identification of linear B-cell epitopes and entry-blocking peptide of severe acute respiratory syndrome (SARS)-associated coronavirus using synthetic overlapping peptide library

Hongbo Hu et al. J Comb Chem. 2005 Sep-Oct.

Abstract

A 10-mer overlapping peptide library has been synthesized for screening and identification of linear B-cell epitopes of severe acute respiratory syndrome associated coronavirus (SARS-CoV), which spanned the major structural proteins of SARS-CoV. One hundred and eleven candidate peptides were positive according to the result of PEPscan, which were assembled into 22 longer peptides. Five of these peptides showed high cross-immunoreactivities (approximately 66.7 to 90.5%) to SARS convalescent patients' sera from the severest epidemic regions of the China mainland. Most interestingly, S(471-503), a peptide located at the receptor binding domain (RBD) of SARS-CoV, could specifically block the binding between the RBD and angiotensin-converting enzyme 2, resulting in the inhibition of SARS-CoV entrance into host cells in vitro. The study demonstrated that S(471-503) peptide was a potential immunoantigen for the development of peptide-based vaccine or a candidate for further drug evaluation against the SARS-CoV virus-cell fusion.

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