Binary malachite green aptamer for fluorescent detection of nucleic acids
- PMID: 16144363
- DOI: 10.1021/ja0529788
Binary malachite green aptamer for fluorescent detection of nucleic acids
Abstract
A new probe that can fluorescently report the presence of specific nucleic acids in solution with extremely high selectivity was developed. The probe consists of malachite green-a triphenylmethane dye-and two short RNA strands, each of which comprises a fragment complementary to an analyte molecule and a fragment of a malachite green aptamer (MGA). The two RNA strands form MGA upon hybridization to the adjacent positions of the nucleic acid analyte. MGA is able to bind malachite green and enhance the fluorescence of the dye, thus monitoring the presence of the nucleic acid in solution. The probe reliably discriminates against 41 out of 42 possible single nucleotide substitutions in 14-mer DNA analyte at room temperature in physiological buffer. Consisting of unmodified RNA strands, which can be expressed in living cells, binary MGA probe represents a promising instrument for real-time nucleic acid monitoring in vivo.
Similar articles
-
A binary DNA probe for highly specific nucleic Acid recognition.J Am Chem Soc. 2006 Aug 16;128(32):10625-8. doi: 10.1021/ja0628093. J Am Chem Soc. 2006. PMID: 16895431
-
Aptamers switch on fluorescence of triphenylmethane dyes.J Am Chem Soc. 2003 Dec 3;125(48):14716-7. doi: 10.1021/ja037994o. J Am Chem Soc. 2003. PMID: 14640641
-
Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription.Bioorg Med Chem Lett. 2008 Aug 15;18(16):4562-5. doi: 10.1016/j.bmcl.2008.07.040. Epub 2008 Jul 15. Bioorg Med Chem Lett. 2008. PMID: 18667307
-
Binary probes for nucleic acid analysis.Chem Rev. 2010 Aug 11;110(8):4709-23. doi: 10.1021/cr900323b. Chem Rev. 2010. PMID: 20583806 Review. No abstract available.
-
Nucleic acid fluorescent probes for biological sensing.Appl Spectrosc. 2012 Nov;66(11):1249-62. doi: 10.1366/12-06803. Appl Spectrosc. 2012. PMID: 23146180 Review.
Cited by
-
RNA plasticity and selectivity applicable to therapeutics and novel biosensor development.Genes Cells. 2012 May;17(5):344-64. doi: 10.1111/j.1365-2443.2012.01596.x. Epub 2012 Apr 4. Genes Cells. 2012. PMID: 22487172 Free PMC article. Review.
-
Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription.Biosensors (Basel). 2023 Mar 31;13(4):449. doi: 10.3390/bios13040449. Biosensors (Basel). 2023. PMID: 37185524 Free PMC article.
-
DNA/RNA Detection Using DNA-Templated Few-Atom Silver Nanoclusters.Biosensors (Basel). 2013 Apr 23;3(2):185-200. doi: 10.3390/bios3020185. Biosensors (Basel). 2013. PMID: 25586126 Free PMC article. Review.
-
A universal method for sensitive and cell-free detection of CRISPR-associated nucleases.Chem Sci. 2019 Jan 16;10(9):2653-2662. doi: 10.1039/c8sc03426e. eCollection 2019 Mar 7. Chem Sci. 2019. PMID: 30996981 Free PMC article.
-
Fluorescence detection of single-nucleotide polymorphisms with a single, self-complementary, triple-stem DNA probe.Angew Chem Int Ed Engl. 2009;48(24):4354-8. doi: 10.1002/anie.200900369. Angew Chem Int Ed Engl. 2009. PMID: 19431180 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
