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Comparative Study
. 2005 Jul 21;47(2):201-13.
doi: 10.1016/j.neuron.2005.06.007.

Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum

Mikio Hoshino  1 , Shoko Nakamura, Kiyoshi Mori, Takeshi Kawauchi, Mami Terao, Yoshiaki V Nishimura, Akihisa Fukuda, Toshimitsu Fuse, Naoki Matsuo, Masaki Sone, Masahiko Watanabe, Haruhiko Bito, Toshio Terashima, Christopher V E Wright, Yoshiya Kawaguchi, Kazuwa Nakao, Yo-Ichi Nabeshima
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Free article
Comparative Study

Ptf1a, a bHLH transcriptional gene, defines GABAergic neuronal fates in cerebellum

Mikio Hoshino et al. Neuron. .
Free article

Abstract

The molecular machinery governing glutamatergic-GABAergic neuronal subtype specification is unclear. Here we describe a cerebellar mutant, cerebelless, which lacks the entire cerebellar cortex in adults. The primary defect of the mutant brains was a specific inhibition of GABAergic neuron production from the cerebellar ventricular zone (VZ), resulting in secondary and complete loss of external germinal layer, pontine, and olivary nuclei during development. We identified the responsible gene, Ptf1a, whose expression was lost in the cerebellar VZ but was maintained in the pancreas in cerebelless. Lineage tracing revealed that two types of neural precursors exist in the cerebellar VZ: Ptf1a-expressing and -nonexpressing precursors, which generate GABAergic and glutamatergic neurons, respectively. Introduction of Ptf1a into glutamatergic neuron precursors in the dorsal telencephalon generated GABAergic neurons with representative morphological and migratory features. Our results suggest that Ptf1a is involved in driving neural precursors to differentiate into GABAergic neurons in the cerebellum.

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