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. 2005 Jun;43(6):2756-63.
doi: 10.1128/JCM.43.6.2756-2763.2005.

Central European Dobrava Hantavirus isolate from a striped field mouse (Apodemus agrarius)

Boris Klempa  1 , Michal Stanko, Milan Labuda, Rainer Ulrich, Helga Meisel, Detlev H Krüger
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Central European Dobrava Hantavirus isolate from a striped field mouse (Apodemus agrarius)

Boris Klempa et al. J Clin Microbiol. 2005 Jun.

Abstract

Dobrava virus (DOBV) is a hantavirus that causes hemorrhagic fever with renal syndrome (HFRS) in Europe. It is hosted by at least two rodent species, Apodemus flavicollis and A. agrarius. According to their natural hosts they form the distinct genetic lineages DOBV-Af and DOBV-Aa, respectively. We have now established a DOBV isolate named Slovakia (SK/Aa) from an A. agrarius animal captured in Slovakia. The complete S and M and partial L segment nucleotide sequences of the new isolate were determined. Phylogenetic analyses showed that the SK/Aa isolate clustered together with the other DOBV-Aa sequences amplified from A. agrarius before and can be taken as the representative of this genetic lineage. SK/Aa, in comparison with a DOBV-Af isolate, was used for serotyping neutralizing antibodies of HFRS patients in Central Europe. Most patients' sera exhibited a higher endpoint titer when probed with our new isolate, suggesting that DOBV-Aa strains are responsible for most of the DOBV-caused HFRS cases in this region.

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Figures

FIG. 1.
FIG. 1.
ML phylogenetic trees of DOBV strains based on complete S-segment (a) and complete M-segment (b) ORF nucleotide sequences corresponding to S-segment nucleotide sequence positions 36 to 1,325 and M-segment nucleotide sequence positions 41 to 3,445, respectively, in SK/Aa, and partial L-segment sequences (374 nt, positions 157 to 530) (c). Names of DOBV isolates are in boldface. Different DOBV clades are indicated by gray boxes. For abbreviations and accession numbers, see the Materials and Methods. The trees were computed with the TREE-PUZZLE package. The values at the tree branches are the PUZZLE support values.
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