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. 2005 Jun;4(6):1155-7.
doi: 10.1128/EC.4.6.1155-1157.2005.

Small sense and antisense RNAs derived from a telomeric retroposon family in Giardia intestinalis

Elisabetta Ullu  1 , Hugo D Lujan, Christian Tschudi
Affiliations

Small sense and antisense RNAs derived from a telomeric retroposon family in Giardia intestinalis

Elisabetta Ullu et al. Eukaryot Cell. 2005 Jun.

Abstract

Sequencing of a library of small RNAs from Giardia intestinalis identified a novel class of small sense and antisense RNAs homologous to the retroposon family GilT/Genie1 that is located at certain telomeres. These small RNAs may contribute to silencing GilT expression via the RNA interference pathway.

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Figures

FIG. 1.
FIG. 1.
(A) Schematic representation of the GilT element and distribution of small sense and antisense GilT tags. Sense and antisense tags are indicated as dashes above and below the GilT drawing, respectively. The sizes and positions of the tags are indicative only and not to scale (for precise locations and sequences of the tags, see Fig. S1 in the supplemental material). The positions of the probes used in the Northern blots are indicated. UTR, untranslated region. (B) Identification of GilT small RNAs in G. intestinalis. Total RNA from G. intestinalis was enriched for small RNAs as described previously (25). Two different RNA samples (lanes 1 and 2) were fractionated on a 15% sequencing gel and analyzed by Northern blotting with a radiolabeled sense riboprobe mixture covering the regions indicated in panel A. M, end-labeled MspI-digested pBR322 molecular weight marker. (C) Large transcripts from GilT elements. Total Giardia RNA (10 μg) from trophozoites was fractionated by electrophoresis on a 1.2% agarose-formaldehyde gel and analyzed by Northern blotting with a sense or antisense radiolabeled GilT probe prepared by asymmetric PCR from the regions indicated in panel A.
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