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. 2005 Jun 14;44(23):8230-43.
doi: 10.1021/bi050372q.

Secondary structure formation of a transmembrane segment in Kv channels

Jianli Lu  1 , Carol Deutsch
Affiliations

Secondary structure formation of a transmembrane segment in Kv channels

Jianli Lu et al. Biochemistry. .

Abstract

Transmembrane segments in the intact voltage-gated potassium (Kv) channel are helical. To ascertain whether this helicity could first be manifested inside the ribosomal tunnel, we generated biogenic peptide intermediates of Kv1.3 and mass-tagged the cysteine-scanned S6 transmembrane segment using pegylation (PEG-MAL) and calmodulation (CaM-MAL). For reference, we created an extended peptide that was used as a "molecular tape measure" of the ribosomal tunnel and determined that the functional length of the tunnel is 99-112 A. We demonstrate that the S6 segment forms a compact structure inside the ribosomal tunnel and that the N-terminal half of S6 compacts more than the C-terminal half of S6. These results bear on the earliest folding events during biogenesis of ion channels.

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