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. 2005 May;11(5):649-54.
doi: 10.3201/eid1105.041211.

Adenovirus type 7 peptide diversity during outbreak, Korea, 1995-2000

Eun Hwa Choi  1 , Hee Sup Kim, Byung Wook Eun, Beyong Il Kim, Jung Yeon Choi, Hoan Jong Lee, Toshiki Inada
Affiliations

Adenovirus type 7 peptide diversity during outbreak, Korea, 1995-2000

Eun Hwa Choi et al. Emerg Infect Dis. 2005 May.

Erratum in

  • Emerg Infect Dis. 2005 Jul;11(6):976

Abstract

To understand the molecular basis of observed regional shifts in the genome types of adenovirus type 7 (Ad7) isolated in Korea during nationwide outbreaks from 1995 to 2000, the genetic variabilities of Ad7d and Ad7l were studied by sequence analysis of hexon, fiber, E3, and E4 open reading frame (ORF) 6/7 peptides. One amino acid change in the receptor-binding domain of fiber and 6 amino acid variations in E4 ORF 6/7 were identified between 2 genome types, while no variations were found in hexon and E3. Phylogenetic trees based on hexon, fiber, and E4 suggested that the Ad7 epidemic was probably caused by the introduction of the Japanese Ad7d strains. Our data also provide evidence that the rapid divergence of Ad7d to a novel genome type Ad7l could have been due to viral strategies involving multiple sequence changes in E4. This result suggests fiber and E4 ORF 6/7 peptides participate in the evolution of Ad7.

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Figures

Figure 1
Figure 1
Schematic representation of the restriction mapping sites of adenovirus type 7d (Ad7d) and Ad7l by BamHI, the primer sequences, and their location for the sequencing analysis of hexon, E3, fiber, and E4 open reading frame (ORF) 6/7 peptides. Each restriction site by BamHI is indicated as (↑). The restriction site at 0.93 map units shown by (↑*) is lost in strains with genome type Ad7l and present in Ad7d. Figure of each primer represents H, hexon; E, E3, fiber; and E4 ORF 6/7 peptides, the position of the first nucleotide at the 5´ end; f, forward; and r, reverse. The numbering of nucleotides is based on that of strain 383, which was isolated in Japan in 1992 (AF053086 for hexon and AF104383 for E3, fiber, and E4).
Figure 2
Figure 2
Phylogenetic analyses of both hexon and fiber with E4 open reading frame (ORF) 6/7 peptides in adenovirus type 7 (Ad7). Phylogenetic trees were constructed by using the neighbor-joining algorithm. Branch lengths are proportional to the number of nucleotide substitutes, and bootstrap probabilities ≥60 are shown at each adjacent node. A) Phylogenetic tree based on 1,428 bp making up nucleotides 297–1725 of the hexon gene of 17 strains of Ad7 with diverse genome types. The sequence of Ad3 (X76549) was defined as an outgroup. B) Phylogenetic tree based on 2,150 bp, including the complete sequence of fiber and E4 ORF 6/7 peptides.
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