The small G-protein Arf6GTP recruits the AP-2 adaptor complex to membranes
- PMID: 15802264
- DOI: 10.1074/jbc.M503099200
The small G-protein Arf6GTP recruits the AP-2 adaptor complex to membranes
Abstract
The small GTP-binding protein ADP-ribosylation factor 6 (Arf6) is involved in plasma membrane/endosomes trafficking. However, precisely how the activation of Arf6 regulates vesicular transport is still unclear. Here, we show that, in vitro, recombinant Arf6GTP recruits purified clathrin-adaptor complex AP-2 (but not AP-1) onto phospholipid liposomes in the absence of phosphoinositides. We also show that phosphoinositides and Arf6 tightly cooperate to translocate AP-2 to the membrane. In vivo, Arf6GTP (but not Arf6GDP) was found associated to AP-2. The expression of the GTP-locked mutant of Arf6 leads to the plasma membrane redistribution of AP-2 in Arf6GTP-enriched areas. Finally, we demonstrated that the expression of the GTP-locked mutant of Arf6 inhibits transferrin receptor internalization without affecting its recycling. Altogether, our results demonstrated that Arf6GTP interacts specifically with AP-2 and promotes its membrane recruitment. These findings strongly suggest that Arf6 plays a major role in clathrin-mediated endocytosis by directly controlling the assembly of the AP-2/clathrin coat.
Similar articles
-
ARF6 regulates angiotensin II type 1 receptor endocytosis by controlling the recruitment of AP-2 and clathrin.Cell Signal. 2007 Nov;19(11):2370-8. doi: 10.1016/j.cellsig.2007.07.015. Epub 2007 Jul 31. Cell Signal. 2007. PMID: 17719203
-
ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma.J Cell Biol. 2003 Jul 7;162(1):113-24. doi: 10.1083/jcb.200301006. J Cell Biol. 2003. PMID: 12847086 Free PMC article.
-
ADP-Ribosylation factor 1 (ARF1) regulates recruitment of the AP-3 adaptor complex to membranes.J Cell Biol. 1998 Jul 27;142(2):391-402. doi: 10.1083/jcb.142.2.391. J Cell Biol. 1998. PMID: 9679139 Free PMC article.
-
Arf6 negatively controls the rapid recycling of the β2 adrenergic receptor.J Cell Sci. 2012 Sep 1;125(Pt 17):4026-35. doi: 10.1242/jcs.102343. Epub 2012 May 18. J Cell Sci. 2012. PMID: 22611259
-
Involvement of a novel ADP-ribosylation factor GTPase-activating protein, SMAP, in membrane trafficking: implications in cancer cell biology.Cancer Sci. 2006 Sep;97(9):801-6. doi: 10.1111/j.1349-7006.2006.00251.x. Epub 2006 Jun 29. Cancer Sci. 2006. PMID: 16805823 Free PMC article. Review.
Cited by
-
Structural basis for recruitment and activation of the AP-1 clathrin adaptor complex by Arf1.Cell. 2013 Feb 14;152(4):755-67. doi: 10.1016/j.cell.2012.12.042. Cell. 2013. PMID: 23415225 Free PMC article.
-
Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11934-9. doi: 10.1073/pnas.0510306103. Epub 2006 Jul 31. Proc Natl Acad Sci U S A. 2006. PMID: 16880396 Free PMC article.
-
A large-scale conformational change couples membrane recruitment to cargo binding in the AP2 clathrin adaptor complex.Cell. 2010 Jun 25;141(7):1220-9. doi: 10.1016/j.cell.2010.05.006. Cell. 2010. PMID: 20603002 Free PMC article.
-
Limiting transport steps and novel interactions of Connexin-43 along the secretory pathway.Histochem Cell Biol. 2009 Sep;132(3):263-80. doi: 10.1007/s00418-009-0617-x. Epub 2009 Jul 22. Histochem Cell Biol. 2009. PMID: 19626334 Free PMC article.
-
The Small GTPase Arf6 Functions as a Membrane Tether in a Chemically-Defined Reconstitution System.Front Cell Dev Biol. 2021 Jan 28;9:628910. doi: 10.3389/fcell.2021.628910. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 33585484 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
