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. 2005 May 6;308(5723):833-8.
doi: 10.1126/science.1109020. Epub 2005 Mar 17.

MicroRNAs regulate brain morphogenesis in zebrafish

Antonio J Giraldez  1 , Ryan M Cinalli, Margaret E Glasner, Anton J Enright, J Michael Thomson, Scott Baskerville, Scott M Hammond, David P Bartel, Alexander F Schier
Affiliations
Free article

MicroRNAs regulate brain morphogenesis in zebrafish

Antonio J Giraldez et al. Science. .
Free article

Abstract

MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects in MZdicer mutants, revealing essential roles for miRNAs during morphogenesis.

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