Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2005 Mar:(432):242-51.
doi: 10.1097/01.blo.0000149812.32857.8b.

Selective retention of bone marrow-derived cells to enhance spinal fusion

George F Muschler  1 , Yoichi Matsukura, Hironori Nitto, Cynthia A Boehm, Antonio D Valdevit, Helen E Kambic, William J Davros, Kirk A Easley, Kimerly A Powell
Affiliations

Selective retention of bone marrow-derived cells to enhance spinal fusion

George F Muschler et al. Clin Orthop Relat Res. 2005 Mar.

Abstract

Connective tissue progenitors can be concentrated rapidly from fresh bone marrow aspirates using some porous matrices as a surface for cell attachment and selective retention, and for creating a cellular graft that is enriched with respect to the number of progenitor cells. We evaluated the potential value of this method using demineralized cortical bone powder as the matrix. Matrix alone, matrix plus marrow, and matrix enriched with marrow cells were compared in an established canine spinal fusion model. Fusions were compared based on union score, fusion mass, fusion volume, and by mechanical testing. Enriched matrix grafts delivered a mean of 2.3 times more cells and approximately 5.6 times more progenitors than matrix mixed with bone marrow. The union score with enriched matrix was superior to matrix alone and matrix plus marrow. Fusion volume and fusion area also were greater with the enriched matrix. These data suggest that the strategy of selective retention provides a rapid, simple, and effective method for concentration and delivery of marrow-derived cells and connective tissue progenitors that may improve the outcome of bone grafting procedures in various clinical settings.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. American Association of Tissue Banks . American Association of Tissue Banks; McLean, VA: 1998. Standards for Tissue Banking.
    1. Bauer TW, Muschler GF. Bone graft materials: An overview of the basic science. Clin Orthop. 2000;371:10–27. - PubMed
    1. Boden SD, Martin GJ, Jr, Morone M. The use of coralline hydroxyapatite with bone marrow, autogenous bone graft, or osteo-inductive bone protein extract for posterolateral lumbar spine fusion. Spine. 1999;24:320–327. - PubMed
    1. Bone Grafts and Bone Substitutes Orthopaedic Network News. 2000;11:8–9.
    1. Burwell RG. The function of bone marrow in the incorporation of a bone graft. Clin Orthop. 1985;200:125–141. - PubMed

Publication types