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. 2005 Mar;149(3):227-34.
doi: 10.1016/j.jsb.2004.10.006.

TOM software toolbox: acquisition and analysis for electron tomography

Stephan Nickell  1 , Friedrich Förster, Alexandros Linaroudis, William Del Net, Florian Beck, Reiner Hegerl, Wolfgang Baumeister, Jürgen M Plitzko
Affiliations

TOM software toolbox: acquisition and analysis for electron tomography

Stephan Nickell et al. J Struct Biol. 2005 Mar.

Abstract

Automated data acquisition procedures have changed the perspectives of electron tomography (ET) in a profound manner. Elaborate data acquisition schemes with autotuning functions minimize exposure of the specimen to the electron beam and sophisticated image analysis routines retrieve a maximum of information from noisy data sets. "TOM software toolbox" integrates established algorithms and new concepts tailored to the special needs of low dose ET. It provides a user-friendly unified platform for all processing steps: acquisition, alignment, reconstruction, and analysis. Designed as a collection of computational procedures it is a complete software solution within a highly flexible framework. TOM represents a new way of working with the electron microscope and can serve as the basis for future high-throughput applications.

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