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. 2005 Jun 1;105(11):4282-9.
doi: 10.1182/blood-2004-02-0756. Epub 2005 Feb 17.

Identification and characterization of undifferentiated mast cells in mouse bone marrow

Maria Célia Jamur  1 , Ana Cristina G Grodzki, Elsa H Berenstein, Majed M Hamawy, Reuben P Siraganian, Constance Oliver
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Free article

Identification and characterization of undifferentiated mast cells in mouse bone marrow

Maria Célia Jamur et al. Blood. .
Free article

Abstract

Sequential immunomagnetic isolation with 2 monoclonal antibodies was used to purify and characterize an undifferentiated mast cell in adult mouse bone marrow that had not been previously recognized. This cell represents 0.02% of the cells in the bone marrow, is CD34(+), CD13(+), and c-kit(+), and does not express FcepsilonRI. However, by polymerase chain reaction (PCR) the cell contains message for the alpha and beta subunits of FcepsilonRI, mast cell-specific proteases, and carboxypeptidase A. Morphologically, this cell has a large nucleus, little cytoplasm, few cytoplasmic organelles, and no cytoplasmic granules. In vitro, in the presence of interleukin-3 (IL-3) and stem cell factor (SCF) these cells differentiate only into a granulated mast cell that now expresses CD13, c-kit, mast cell-specific gangliosides, FcepsilonRI, and binds immunoglobulin E (IgE). When injected into lethally irradiated mice, these cells are able to reconstitute the mast cell population in the spleen.

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