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. 2004 Dec;10(12):2228-30.
doi: 10.3201/eid1012.040452.

Isolation and molecular identification of Nipah virus from pigs

Sazaly AbuBakar  1 , Li-Yen Chang, A R Mohd Ali, S H Sharifah, Khatijah Yusoff, Zulkeflie Zamrod
Affiliations

Isolation and molecular identification of Nipah virus from pigs

Sazaly AbuBakar et al. Emerg Infect Dis. 2004 Dec.

Abstract

Nipah viruses from pigs from a Malaysian 1998 outbreak were isolated and sequenced. At least two different Nipah virus strains, including a previously unreported strain, were identified. The findings highlight the possibility that the Malaysia outbreaks had two origins of Nipah virus infections.

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Figures

Figure
Figure
Phylogenetic trees illustrating the relationships of the pig Nipah virus isolates to all other known Nipah viruses and the related members of subfamily Paramyxovirinae. A) The maximum likelihood tree was drawn by using alignments of the full genome sequences. All the new isolates described in the study are shown in boxes. Abbreviations used and accession numbers not described elsewhere in the text are in parenthesis: Tupaia paramyxovirus (Tupaia-A) (AF079780); (Tupaia-N) (NC_002199), and Hendra virus (HV-A) (AF017149); (HV-N) (NC_001906). B) Unrooted maximum likelihood plot was constructed by using alignments of all the nucleotide differences in the Nipah virus gene coding regions (N, P, M, F, and G) shown in Table A1, Table A2, and Table A3 by artificially treating all the differences as a single stretch of nucleotide sequence.
See this image and copyright information in PMC

Comment in

  • Nipah virus strain variation.
    Pulliam JR, Field HE, Olival KJ; Henipavirus Ecology Research Group. Pulliam JR, et al. Emerg Infect Dis. 2005 Dec;11(12):1978-9; author reply 1979. doi: 10.3201/eid1112.050220. Emerg Infect Dis. 2005. PMID: 16485499 Free PMC article. No abstract available.

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