Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Dec;10(12):2189-91.
doi: 10.3201/eid1012.040759.

Avian influenza H5N1 in tigers and leopards

Juthatip Keawcharoen  1 , Kanisak Oraveerakul, Thijs Kuiken, Ron A M Fouchier, Alongkorn Amonsin, Sunchai Payungporn, Suwanna Noppornpanth, Sumitra Wattanodorn, Apiradee Theambooniers, Rachod Tantilertcharoen, Rattapan Pattanarangsan, Nlin Arya, Parntep Ratanakorn, D M E Osterhaus, Yong Poovorawan
Affiliations

Avian influenza H5N1 in tigers and leopards

Juthatip Keawcharoen et al. Emerg Infect Dis. 2004 Dec.

Abstract

Influenza virus is not known to affect wild felids. We demonstrate that avian influenza A (H5N1) virus caused severe pneumonia in tigers and leopards that fed on infected poultry carcasses. This finding extends the host range of influenza virus and has implications for influenza virus epidemiology and wildlife conservation.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Histopathologic and immunohistochemical evidence of avian influenza A (H5N1) virus in leopard lung. A) Diffuse alveolar damage in the lung: alveoli and bronchioles (between arrowheads) are flooded with edema fluid and inflammatory cells. B) Inflammatory cells in alveolar lumen consist of alveolar macrophages (arrowhead) and neutrophils (arrow). C) Many cells in affected lung tissue express influenza virus antigen, visible as brown staining. D) Expression of influenza virus antigen in a bronchiole is visible mainly in nuclei of epithelial cells.
Figure 2
Figure 2
Phylogenetic comparison of zoo felid isolates with other H5N1 viruses. DNA maximum likelihood tree of hemagglutinin and neuraminidase sequences. Representative full-length Asian influenza A virus H5 (A) and N1 (B) sequences from 1996 to 2004 are shown with 2004 sequences in bold and leopard and tiger sequences underlined. Maximum likelihood trees were generated by using 100 bootstraps and three jumbles, and the resulting consensus trees were used as a user tree to recalculate branch lengths. The trees had good bootstrap support. Scale bars roughly indicate 1% nucleotide difference between related strains. Accession no. used: A/Goose/Guangdong/1/1996 (AF144305 and AF144304), A/Hong Kong/156/1997 (AF028709 and AF028708), A/Goose/Hong Kong/ww491/2000 (AY059480 and AY059489), A/Goose/Hong Kong/ww28/2000 (AY059475 and AY059484), A/Chicken/Hong Kong/YU562/2001 (AY221529 and AY221547), A/Duck/Hong Kong/2986.1/2000 (AY059481 and AY059490), A/Goose/Hong Kong/3014.8/2000 (AY059482 and AY059491), A/duck/China/E319-2/2003 (AY518362 and AY518363), A/Thailand/1-KAN-1/2004 (AY555150 and AY555151), A/Thailand/2-SP-33/2004 (AY555153 and AY555152), A/Chicken/Thailand/CU-K2/2004 (AY590568 and AY590567), A/Leopard/Thailand/2004 (AY646175 and AY646176), and A/Tiger/Thailand/2004 (AY646167 and AY646168).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cases of influenza A (H5N1)—Thailand, 2004. MMWR Morb Mortal Wkly Rep. 2004;53:100–3. - PubMed
    1. Poddar SK. Influenza virus types and subtypes detection by single step single tube multiplex reverse transcription-polymerase chain reaction (RT-PCR) and agarose gel electrophoresis. J Virol Methods. 2002;99:63–70. 10.1016/S0166-0934(01)00380-9 - DOI - PubMed
    1. Rimmelzwaan GF, Kuiken T, van Amerongen G, Bestebroer TM, Fouchier RAM, Osterhaus ADME. Pathogenesis of influenza A (H5N1) virus infection in a primate model. J Virol. 2001;75:6687–91. 10.1128/JVI.75.14.6687-6691.2001 - DOI - PMC - PubMed
    1. Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR. Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol. 2001;146:2275–89. 10.1007/s007050170002 - DOI - PubMed
    1. Felsenstein J. PHYLIP—phylogeny inference package (version 3.2). Cladistics. 1989;5:164–6.

Publication types

LinkOut - more resources