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Comparative Study
. 1992 Apr 15;89(8):3536-40.
doi: 10.1073/pnas.89.8.3536.

RNA polymerase-associated transcription specificity factor encoded by vaccinia virus

B Y Ahn  1 , B Moss
Affiliations
Comparative Study

RNA polymerase-associated transcription specificity factor encoded by vaccinia virus

B Y Ahn et al. Proc Natl Acad Sci U S A. .

Abstract

Vaccinia virus encodes a multisubunit DNA-dependent RNA polymerase (EC 2.7.7.6) that is packaged in the infectious virus particle. This polymerase was found to contain a submolar polypeptide of approximately 85 kDa in addition to the core subunits, which consist of two larger and several smaller polypeptides. The polymerase containing the 85-kDa polypeptide was separated from the core polymerase by column chromatography. Although the core polymerase actively transcribed heterologous single-stranded DNA, only the form with the associated 85-kDa polypeptide could act in conjunction with an early stage-specific factor to transcribe double-stranded DNA containing a vaccinia virus early promoter. Peptide sequencing established that the RNA polymerase-associated 85-kDa protein was derived from the vaccinia virus H4L open reading frame, which encodes a 94-kDa polypeptide that we named RAP94. RAP94 is not closely related to prokaryotic sigma 70 or eukaryotic RAP30 RNA polymerase-binding proteins, although there are short regions of sequence similarity. The specific association of RAP94 with viral RNA polymerase was corroborated with antibody raised to a recombinant fusion protein. Unlike the previously defined subunits of vaccinia virus RNA polymerase, RAP94 is synthesized exclusively late in infection, and synthesis could be prevented by a DNA replication inhibitor. The role of RAP94 in mediating specific transcription was demonstrated by using an extract from cells in which the H4L open reading frame had been transiently expressed.

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References

    1. J Biol Chem. 1977 Oct 10;252(19):6930-8 - PubMed
    1. J Biol Chem. 1980 May 10;255(9):4372-80 - PubMed
    1. Virology. 1991 Jan;180(1):88-98 - PubMed
    1. J Biol Chem. 1991 Jul 25;266(21):13712-8 - PubMed
    1. J Biol Chem. 1991 Jan 25;266(3):1355-8 - PubMed

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