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Comparative Study
. 2005 Jan;12(1):101-6.
doi: 10.1128/CDLI.12.1.101-106.2005.

CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculin is an indicator of immune sensitization against Mycobacterium tuberculosis antigens

Bojana Avgustin  1 , Vladimir Kotnik, Mojca Skoberne, Tadej Malovrh, Aleksandra Skralovnik-Stern, Marjeta Tercelj
Affiliations
Comparative Study

CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculin is an indicator of immune sensitization against Mycobacterium tuberculosis antigens

Bojana Avgustin et al. Clin Diagn Lab Immunol. 2005 Jan.

Abstract

The expression of the CD69 antigen on CD4 T lymphocytes after in vitro stimulation with purified protein derivative (2 tuberculin units) was used to evaluate the tuberculin reactivities of 52 individuals from four experimental groups: Mycobacterium bovis BCG-vaccinated healthy individuals with a negative tuberculin skin test (TST) result (group A), BCG-vaccinated healthy individuals with a positive TST result (group B), patients with active tuberculosis (TB) before treatment (group C), and individuals with clinically inactive TB who had previously completed a prescribed course of chemotherapy (group D). The expression of CD69 on CD4 T lymphocytes was significantly higher in patients with active TB (16.2%+/-7.3%), individuals with clinically inactive TB (10.5%+/-7.4%), and healthy individuals with a positive TST result (15.5%+/-7.2%) than in healthy individuals with a negative TST result (3.8%+/-4.3%) (P<0.005). We confirmed the correlation between CD69 antigen expression on T lymphocytes after stimulation with tuberculin and the TST induration diameter (Spearman rho=0.783; P<0.001), an assay for gamma interferon (the Quantiferon-TB assay; Spearman rho=0.613; P<0.001), and the lymphocyte BLAST transformation test (Spearman rho=0.537; P<0.001). Our results demonstrate the usefulness of the determination of CD69 on CD4 T lymphocytes after in vitro stimulation with tuberculin as a rapid indicator of immune sensitization against Mycobacterium tuberculosis.

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Figures

FIG. 1.
FIG. 1.
CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculin (upper left), the QFT response (upper right), and the LTT result (lower left) for whole-blood samples from BCG-vaccinated healthy individuals with a negative TST reaction (group A), BCG-vaccinated healthy individuals with a positive TST reaction (group B), patients with active TB before treatment (group C), and individuals with clinically inactive TB (group D). Circles, individual values; lines, mean value for the group.
FIG. 1.
FIG. 1.
CD69 expression on CD4+ T lymphocytes after in vitro stimulation with tuberculin (upper left), the QFT response (upper right), and the LTT result (lower left) for whole-blood samples from BCG-vaccinated healthy individuals with a negative TST reaction (group A), BCG-vaccinated healthy individuals with a positive TST reaction (group B), patients with active TB before treatment (group C), and individuals with clinically inactive TB (group D). Circles, individual values; lines, mean value for the group.
FIG. 2.
FIG. 2.
Scattergram showing the correlation between CD69 expression on CD4+ T lymphocytes in whole-blood samples stimulated with tuberculin and the TST induration diameter for groups of healthy individuals with a negative TST reaction (group A; open circles) and a positive TST reaction (group B; closed circles).
See this image and copyright information in PMC

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