Human monocyte CD163 expression inversely correlates with soluble CD163 plasma levels
- PMID: 15624200
- DOI: 10.1002/cyto.b.20031
Human monocyte CD163 expression inversely correlates with soluble CD163 plasma levels
Abstract
Background: CD163 is a monocyte/macrophage-restricted receptor involved in the clearance of hemoglobin-haptoglobin complexes and regulation of inflammatory processes. CD163 is shed from the cell surface and exists as a soluble form in plasma (sCD163). Monocyte CD163 and sCD163 are potential diagnostic tools in variety of disease states.
Methods: We determined the relation between plasma sCD163 levels by enzyme-linked immunosorbent assay, membrane expressions of CD163, CD64, and CD14 on blood monocytes by flow cytometry, and monocyte counts in 129 random blood samples.
Results: A strong inverse correlation was found between membrane CD163 expression and sCD163 levels (r = -0.65, P < 0.001). Monocyte CD163 expression and SCD163 levels did not correlate with the monocyte absolute count.
Conclusions: The inverse relation between monocyte surface CD163 expression and sCD163 levels in human blood suggests that plasma sCD163 is derived from circulating monocytes, in addition to an unknown component from tissue macrophages. The lack of correlation with the absolute monocyte number suggests that such a balance is driven by the functional state of monocytes, rather than simply by numerical changes in circulating cells. We propose that further clinical evaluations of CD163 as a diagnostic parameter should include simultaneous measurements of soluble and cell-bound forms of this antigen.
(c) 2004 Wiley-Liss, Inc.
Similar articles
-
Association of CD163+ macrophages and local production of soluble CD163 with decreased lymphocyte activation in spondylarthropathy synovitis.Arthritis Rheum. 2004 May;50(5):1611-23. doi: 10.1002/art.20174. Arthritis Rheum. 2004. PMID: 15146432
-
Proteolytic shedding of the macrophage scavenger receptor CD163 in multiple sclerosis.J Neuroimmunol. 2007 Jul;187(1-2):179-86. doi: 10.1016/j.jneuroim.2007.04.016. Epub 2007 May 29. J Neuroimmunol. 2007. PMID: 17537523
-
Differential expression of monocyte CD163 in single- and dual-asthmatic responders during allergen-induced bronchoconstriction.Clin Exp Allergy. 2006 Dec;36(12):1584-91. doi: 10.1111/j.1365-2222.2006.02573.x. Clin Exp Allergy. 2006. PMID: 17177682
-
Soluble CD163.Scand J Clin Lab Invest. 2012 Feb;72(1):1-13. doi: 10.3109/00365513.2011.626868. Epub 2011 Nov 7. Scand J Clin Lab Invest. 2012. PMID: 22060747 Review.
-
Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy.Mol Immunol. 2010 Apr;47(7-8):1650-60. doi: 10.1016/j.molimm.2010.02.008. Epub 2010 Mar 17. Mol Immunol. 2010. PMID: 20299103 Review.
Cited by
-
Relationship Between sCD163 and mCD163 and Their Implication in the Detection and Typing of Leprosy.Clin Cosmet Investig Dermatol. 2020 Jun 2;13:379-389. doi: 10.2147/CCID.S240420. eCollection 2020. Clin Cosmet Investig Dermatol. 2020. PMID: 32581565 Free PMC article.
-
Differential expression of CD163 on monocyte subsets in healthy and HIV-1 infected individuals.PLoS One. 2011;6(5):e19968. doi: 10.1371/journal.pone.0019968. Epub 2011 May 20. PLoS One. 2011. PMID: 21625498 Free PMC article.
-
CD163ΔSRCR5 MARC-145 Cells Resist PRRSV-2 Infection via Inhibiting Virus Uncoating, Which Requires the Interaction of CD163 With Calpain 1.Front Microbiol. 2020 Jan 13;10:3115. doi: 10.3389/fmicb.2019.03115. eCollection 2019. Front Microbiol. 2020. PMID: 32038556 Free PMC article.
-
Higher levels of circulating monocyte-platelet aggregates are correlated with viremia and increased sCD163 levels in HIV-1 infection.Cell Mol Immunol. 2015 Jul;12(4):435-43. doi: 10.1038/cmi.2014.66. Epub 2014 Aug 11. Cell Mol Immunol. 2015. PMID: 25109683 Free PMC article. Clinical Trial.
-
Effect of Periodic Granulocyte Colony-Stimulating Factor Administration on Endothelial Progenitor Cells and Different Monocyte Subsets in Pediatric Patients with Muscular Dystrophies.Stem Cells Int. 2016;2016:2650849. doi: 10.1155/2016/2650849. Epub 2015 Dec 6. Stem Cells Int. 2016. PMID: 26770204 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
