Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators
- PMID: 1555237
- DOI: 10.1016/0092-8674(92)90124-u
Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators
Abstract
Brefeldin A (BFA) causes a rapid redistribution of coat proteins (e.g., gamma-adaptin) associated with the clathrin-coated vesicles that bud from the trans-Golgi network (TGN), while the clathrin-coated vesicles that bud from the plasma membrane are unaffected. gamma-Adaptin redistributes with the same kinetics as beta-COP, a coat protein associated with the non-clathrin-coated vesicles that bud from the Golgi complex. Upon removal of BFA, however, gamma-adaptin recovers its perinuclear distribution more rapidly. Redistribution of both proteins can be prevented by pretreating cells with AlF4-. Recruitment of adaptors from the cytosol onto the TGN membrane has been reconstituted in a permeabilized cell system and is increased by addition of GTP gamma S and blocked by addition of BFA. These results suggest a role for G proteins in the control of the clathrin-coated vesicle cycle at the TGN and further extend the similarities between clathrin-coated vesicles and non-clathrin-coated vesicles.
Similar articles
-
100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties.J Cell Biol. 1992 Jun;117(6):1171-9. doi: 10.1083/jcb.117.6.1171. J Cell Biol. 1992. PMID: 1607381 Free PMC article.
-
Distinct coated vesicles labeled for p200 bud from trans-Golgi network membranes.Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2874-8. doi: 10.1073/pnas.92.7.2874. Proc Natl Acad Sci U S A. 1995. PMID: 7708740 Free PMC article.
-
Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein.Science. 1991 Nov 22;254(5035):1197-9. doi: 10.1126/science.1957170. Science. 1991. PMID: 1957170
-
Biogenesis of constitutive secretory vesicles, secretory granules and synaptic vesicles.Curr Opin Cell Biol. 1993 Aug;5(4):628-35. doi: 10.1016/0955-0674(93)90132-a. Curr Opin Cell Biol. 1993. PMID: 8257604 Review.
-
Transport and internal organization of membranes: vesicles, membrane networks and GTP-binding proteins.J Cell Sci. 1994 Apr;107 ( Pt 4):753-63. doi: 10.1242/jcs.107.4.753. J Cell Sci. 1994. PMID: 8056835 Review. No abstract available.
Cited by
-
Mammalian Golgi-associated Bicaudal-D2 functions in the dynein-dynactin pathway by interacting with these complexes.EMBO J. 2001 Aug 1;20(15):4041-54. doi: 10.1093/emboj/20.15.4041. EMBO J. 2001. PMID: 11483508 Free PMC article.
-
ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma.J Cell Biol. 2003 Jul 7;162(1):113-24. doi: 10.1083/jcb.200301006. J Cell Biol. 2003. PMID: 12847086 Free PMC article.
-
Neural cell adhesion molecule promotes accumulation of TGN organelles at sites of neuron-to-neuron contacts.J Cell Biol. 2002 Nov 25;159(4):649-61. doi: 10.1083/jcb.200205098. Epub 2002 Nov 18. J Cell Biol. 2002. PMID: 12438412 Free PMC article.
-
GGAs: a family of ADP ribosylation factor-binding proteins related to adaptors and associated with the Golgi complex.J Cell Biol. 2000 Apr 3;149(1):81-94. doi: 10.1083/jcb.149.1.81. J Cell Biol. 2000. PMID: 10747089 Free PMC article.
-
Vesicular trafficking machinery, the actin cytoskeleton, and H+-K+-ATPase recycling in the gastric parietal cell.J Physiol. 2001 Apr 15;532(Pt 2):287-96. doi: 10.1111/j.1469-7793.2001.0287f.x. J Physiol. 2001. PMID: 11306650 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
