A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells
- PMID: 1552184
- DOI: 10.1177/40.4.1552184
A fibroblast-associated antigen: characterization in fibroblasts and immunoreactivity in smooth muscle differentiated stromal cells
Abstract
Fibroblasts with smooth muscle differentiation are frequently derived from human breast tissue. Immunofluorescence cytochemistry of a fibroblast-associated antigen recognized by a monoclonal antibody (MAb), 1B10, was analyzed with a view to discriminating smooth muscle differentiated fibroblasts from vascular smooth muscle cells. The antigen was detected on the cell surface and in cathepsin D-positive and acridine orange-accumulating vesicular compartments of fibroblasts. Ultrastructurally, the antigen was revealed in coated pits and in endosomal and lysosomal structures. 1B10 recognized three major brands migrating at apparent Mr of 38,000, 45,000, and 80,000, in addition to many minor bands between Mr 45,000 and 97,000, including Mr 52,000. The Mr 45,000 and 38,000 were associated with the cell membrane and Mr 52,000 as well as Mr 38,000 were associated with the lysosomes. The 1B10 immunoreactivity was specific to fibroblasts and smooth muscle differentiated fibroblasts within the context of vascular smooth muscle cells.
Similar articles
-
Smooth muscle differentiation in cultured human breast gland stromal cells.Lab Invest. 1990 Oct;63(4):532-43. Lab Invest. 1990. PMID: 2232705
-
Expression of smooth muscle-specific proteins in myoepithelium and stromal myofibroblasts of normal and malignant human breast tissue.Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):999-1003. doi: 10.1073/pnas.90.3.999. Proc Natl Acad Sci U S A. 1993. PMID: 8430113 Free PMC article.
-
Dependence of collagen remodelling on alpha-smooth muscle actin expression by fibroblasts.J Cell Physiol. 1994 Apr;159(1):161-75. doi: 10.1002/jcp.1041590120. J Cell Physiol. 1994. PMID: 8138584
-
Co-localization of immunoreactive forms of calponin with actin cytoskeleton in platelets, fibroblasts, and vascular smooth muscle.J Biochem. 1991 Feb;109(2):311-6. J Biochem. 1991. PMID: 1864842
-
What's new in human wound-healing myofibroblasts?Curr Top Pathol. 1999;93:123-33. doi: 10.1007/978-3-642-58456-5_13. Curr Top Pathol. 1999. PMID: 10339905 Review. No abstract available.
Cited by
-
To create the correct microenvironment: three-dimensional heterotypic collagen assays for human breast epithelial morphogenesis and neoplasia.Methods. 2003 Jul;30(3):247-55. doi: 10.1016/s1046-2023(03)00031-8. Methods. 2003. PMID: 12798139 Free PMC article.
-
Novel method of cell line establishment utilizing fluorescence-activated cell sorting resulting in 6 new head and neck squamous cell carcinoma lines.Head Neck. 2016 Apr;38 Suppl 1(Suppl 1):E459-67. doi: 10.1002/hed.24019. Epub 2015 Jul 14. Head Neck. 2016. PMID: 25677579 Free PMC article.
-
Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells.Proc Natl Acad Sci U S A. 1992 Oct 1;89(19):9064-8. doi: 10.1073/pnas.89.19.9064. Proc Natl Acad Sci U S A. 1992. PMID: 1384042 Free PMC article.
-
The origin of the myofibroblasts in breast cancer. Recapitulation of tumor environment in culture unravels diversity and implicates converted fibroblasts and recruited smooth muscle cells.J Clin Invest. 1995 Feb;95(2):859-73. doi: 10.1172/JCI117736. J Clin Invest. 1995. PMID: 7532191 Free PMC article.
-
A function for filamentous alpha-smooth muscle actin: retardation of motility in fibroblasts.J Cell Biol. 1996 Jul;134(1):67-80. doi: 10.1083/jcb.134.1.67. J Cell Biol. 1996. PMID: 8698823 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
