Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer research
- PMID: 15517017
- DOI: 10.1038/sj.onc.1208028
Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer research
Abstract
The completion of whole-genome sequencing of various model organisms and the recent explosion of new technologies in the field of Functional Genomics and Proteomics is poised to revolutionize the way scientists identify and characterize gene function. One of the most significant advances in recent years has been the application of RNA interference (RNAi) as a means of assaying gene function. In the post-genomic era, advances in the field of cancer biology will rely upon the rapid identification and characterization of genes that regulate cell growth, proliferation, and apoptosis. Significant efforts are being directed towards cancer therapy and devising efficient means of selectively delivering drugs to cancerous cells. In this review, we discuss the promise of integrating genome-wide RNAi screens with proteomic approaches and small-molecule chemical genetic screens, towards improving our ability to understand and treat cancer.
Similar articles
-
Drosophila genome-wide RNAi screens: are they delivering the promise?Cold Spring Harb Symp Quant Biol. 2006;71:141-8. doi: 10.1101/sqb.2006.71.027. Cold Spring Harb Symp Quant Biol. 2006. PMID: 17381290 Review.
-
High-throughput RNAi screen in Drosophila.Methods Mol Biol. 2008;469:163-84. doi: 10.1007/978-1-60327-469-2_13. Methods Mol Biol. 2008. PMID: 19109710
-
Genomics and proteomics approaches in understanding tumor angiogenesis.Expert Rev Mol Diagn. 2007 Mar;7(2):133-47. doi: 10.1586/14737159.7.2.133. Expert Rev Mol Diagn. 2007. PMID: 17331062 Review.
-
RNAi and HTS: exploring cancer by systematic loss-of-function.Oncogene. 2004 Nov 1;23(51):8392-400. doi: 10.1038/sj.onc.1208217. Oncogene. 2004. PMID: 15517021 Review.
-
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila.Nature. 2007 Jul 12;448(7150):151-6. doi: 10.1038/nature05954. Nature. 2007. PMID: 17625558
Cited by
-
Genomic screening with RNAi: results and challenges.Annu Rev Biochem. 2010;79:37-64. doi: 10.1146/annurev-biochem-060408-092949. Annu Rev Biochem. 2010. PMID: 20367032 Free PMC article. Review.
-
E6 and E7 gene silencing results in decreased methylation of tumor suppressor genes and induces phenotype transformation of human cervical carcinoma cell lines.Oncotarget. 2015 Sep 15;6(27):23930-43. doi: 10.18632/oncotarget.4525. Oncotarget. 2015. PMID: 26329329 Free PMC article.
-
RNAi in embryonic stem cells.Stem Cell Rev. 2006;2(1):11-8. doi: 10.1007/s12015-006-0003-1. Stem Cell Rev. 2006. PMID: 17142881 Review.
-
Drosophila as a tool for personalized medicine: a primer.Per Med. 2010 Nov;7(6):621-632. doi: 10.2217/pme.10.65. Per Med. 2010. PMID: 21977050 Free PMC article.
-
CellProfiler: image analysis software for identifying and quantifying cell phenotypes.Genome Biol. 2006;7(10):R100. doi: 10.1186/gb-2006-7-10-r100. Epub 2006 Oct 31. Genome Biol. 2006. PMID: 17076895 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
