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Review
. 2005 Feb 1;385(Pt 3):625-37.
doi: 10.1042/BJ20041361.

Monitoring the formation of dynamic G-protein-coupled receptor-protein complexes in living cells

Kevin D G Pfleger  1 , Karin A Eidne
Affiliations
Review

Monitoring the formation of dynamic G-protein-coupled receptor-protein complexes in living cells

Kevin D G Pfleger et al. Biochem J. .

Abstract

GPCRs (G-protein-coupled receptors) play an extremely important role in transducing extracellular signals across the cell membrane with high specificity and sensitivity. They are central to many of the body's endocrine and neurotransmitter pathways, and are consequently a major drug target. It is now clear that GPCRs interact with a range of proteins, including other GPCRs. Identifying and elucidating the function of such interactions will significantly enhance our understanding of cellular function, with the promise of new and improved pharmaceuticals. Biophysical techniques involving resonance energy transfer, namely FRET (fluorescence resonance energy transfer) and BRET (bioluminescence resonance energy transfer), now enable us to monitor the formation of dynamic GPCR-protein complexes in living cells, in real time. Their use has firmly established the concept of GPCR oligomerization, as well as demonstrating GPCR interactions with GPCR kinases, beta-arrestins, adenylate cyclase and a subunit of an inwardly rectifying K+ channel. The present review examines recent technological advances and experimental applications of FRET and BRET, discussing particularly how they have been adapted to extract an ever-increasing amount of information about the nature, specificity, stoichiometry, kinetics and agonist-dependency of GPCR-protein interactions.

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Figures

Figure 1
Figure 1. Resonance energy transfer
RET occurs between donor and acceptor molecules if they are in close proximity (less than 100 Å), resulting in energy emission from the acceptor at a characteristic wavelength. FRET involves excitation of a donor fluorophore with light (A), whereas BRET occurs when the donor Rluc catalyses the oxidation of coelenterazine to coelenteramide (B). Both of these techniques are particularly suitable for investigating GPCR–protein complexes in living cells, including oligomerization (C). Illustration created by Uli Schmidt (http://www.scigraphico.com.au/).
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