Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein
- PMID: 1549178
- DOI: 10.1038/356344a0
Action of brefeldin A blocked by activation of a pertussis-toxin-sensitive G protein
Abstract
In many mammalian cells brefeldin A interferes with mechanisms that keep the Golgi appartus separate from the endoplasmic reticulum. The earliest effect of brefeldin A is release of the coat protein beta-COP from the Golgi. This release is blocked by pretreatment with GTP-gamma S or AlF4- (ref. 12). The AlF4- ion activates heterotrimeric G proteins but not proteins of the ras superfamily, suggesting that a heterotrimeric G protein might control membrane transfer from the endoplasmic reticulum to the Golgi. We report here that mastoparan, a peptide that activates heterotrimeric G proteins, promotes binding of beta-COP to Golgi membranes in vitro and antagonizes the effect of brefeldin A on beta-COP in perforated cells and on isolated Golgi membranes. This inhibition is greatly diminished if cells are pretreated with pertussis toxin before perforation. Thus, a heterotrimeric G protein of the Gi/Go subfamily regulates association of coat components with Golgi membranes.
Similar articles
-
Binding of the cytosolic p200 protein to Golgi membranes is regulated by heterotrimeric G proteins.J Cell Sci. 1993 Dec;106 ( Pt 4):1239-48. doi: 10.1242/jcs.106.4.1239. J Cell Sci. 1993. PMID: 8126104
-
Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF.Nature. 1992 Nov 26;360(6402):352-4. doi: 10.1038/360352a0. Nature. 1992. PMID: 1448152
-
Binding of ARF and beta-COP to Golgi membranes: possible regulation by a trimeric G protein.Science. 1991 Nov 22;254(5035):1197-9. doi: 10.1126/science.1957170. Science. 1991. PMID: 1957170
-
Coatomers and SNAREs in promoting membrane traffic.Cell. 1993 Nov 19;75(4):593-6. doi: 10.1016/0092-8674(93)90477-8. Cell. 1993. PMID: 8242734 Review. No abstract available.
-
Distribution and role of heterotrimeric G proteins in the secretory pathway of polarized epithelial cells.J Cell Sci Suppl. 1993;17:33-9. doi: 10.1242/jcs.1993.supplement_17.6. J Cell Sci Suppl. 1993. PMID: 8144703 Review.
Cited by
-
Multiple GTP-binding proteins regulate vesicular transport from the ER to Golgi membranes.J Cell Biol. 1992 Dec;119(5):1077-96. doi: 10.1083/jcb.119.5.1077. J Cell Biol. 1992. PMID: 1447289 Free PMC article.
-
Basolateral to apical transcytosis in polarized cells is indirect and involves BFA and trimeric G protein sensitive passage through the apical endosome.J Cell Biol. 1994 Jan;124(1-2):83-100. doi: 10.1083/jcb.124.1.83. J Cell Biol. 1994. PMID: 7905002 Free PMC article.
-
RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport.Mol Biol Cell. 2000 Sep;11(9):3155-68. doi: 10.1091/mbc.11.9.3155. Mol Biol Cell. 2000. PMID: 10982407 Free PMC article.
-
Isolation and characterization of mutant CHO cell lines with compartment-specific resistance to brefeldin A.J Cell Biol. 1994 Jul;126(1):65-75. doi: 10.1083/jcb.126.1.65. J Cell Biol. 1994. PMID: 8027187 Free PMC article.
-
Two distinct populations of ARF bound to Golgi membranes.J Cell Biol. 1993 May;121(4):751-60. doi: 10.1083/jcb.121.4.751. J Cell Biol. 1993. PMID: 8491770 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources