Many paths to many clones: a comparative look at high-throughput cloning methods
- PMID: 15489321
- DOI: 10.1101/gr.2528804
Many paths to many clones: a comparative look at high-throughput cloning methods
Abstract
The creation of genome-scale clone resources is a difficult and costly process, making it essential to maximize the efficiency of each step of clone creation. In this review, we compare the available commercial and open-source recombinational cloning methods with regard to their use in creating comprehensive open reading frame (ORF) clone collections with an emphasis on the properties requisite to use in a high-throughput setting. The most efficient strategy to the creation of ORF clone resources is to build a master clone collection that serves as a quality validated source for producing collections of expression clones. We examine the methods for recombinational cloning available for both the creation of master clones and their conversion into expression clones. Alternative approaches to creating clones involving mixing of cloning methods, including gap-repair cloning, are also explored.
Similar articles
-
Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations.Plant Biotechnol J. 2006 May;4(3):317-24. doi: 10.1111/j.1467-7652.2006.00183.x. Plant Biotechnol J. 2006. PMID: 17147637
-
High-throughput construction of ORF clones for production of the recombinant proteins.Methods Mol Biol. 2009;577:25-39. doi: 10.1007/978-1-60761-232-2_3. Methods Mol Biol. 2009. PMID: 19718506
-
High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system.Protein Expr Purif. 2006 Jun;47(2):562-70. doi: 10.1016/j.pep.2005.11.007. Epub 2005 Dec 5. Protein Expr Purif. 2006. PMID: 16377204
-
From genome to proteome: developing expression clone resources for the human genome.Hum Mol Genet. 2006 Apr 15;15 Spec No 1:R31-43. doi: 10.1093/hmg/ddl048. Hum Mol Genet. 2006. PMID: 16651367 Review.
-
Parallel methods for expression and purification.Methods Enzymol. 2009;463:767-85. doi: 10.1016/S0076-6879(09)63041-X. Methods Enzymol. 2009. PMID: 19892201 Review.
Cited by
-
A series of TA-based and zero-background vectors for plant functional genomics.PLoS One. 2013;8(3):e59576. doi: 10.1371/journal.pone.0059576. Epub 2013 Mar 29. PLoS One. 2013. PMID: 23555713 Free PMC article.
-
Protein interaction platforms: visualization of interacting proteins in yeast.Nat Methods. 2009 Jul;6(7):500-2. doi: 10.1038/nmeth.1337. Epub 2009 May 31. Nat Methods. 2009. PMID: 19483691 Free PMC article.
-
PGASO: A synthetic biology tool for engineering a cellulolytic yeast.Biotechnol Biofuels. 2012 Jul 27;5(1):53. doi: 10.1186/1754-6834-5-53. Biotechnol Biofuels. 2012. PMID: 22839502 Free PMC article.
-
The alliance for cellular signaling plasmid collection: a flexible resource for protein localization studies and signaling pathway analysis.Mol Cell Proteomics. 2007 Mar;6(3):413-24. doi: 10.1074/mcp.M600437-MCP200. Epub 2006 Dec 27. Mol Cell Proteomics. 2007. PMID: 17192258 Free PMC article.
-
High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli.Microb Cell Fact. 2013 Apr 22;12:37. doi: 10.1186/1475-2859-12-37. Microb Cell Fact. 2013. PMID: 23607455 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
