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. 1992 Feb 28;1130(1):78-80.
doi: 10.1016/0167-4781(92)90465-c.

Specific hybridization probes for mouse alpha 2(IX) and alpha 1(X) collagen mRNAs

K Elima  1 , M Metsäranta, J Kallio, M Perälä, I Eerola, S Garofalo, B De Crombrugghe, E Vuorio
Affiliations

Specific hybridization probes for mouse alpha 2(IX) and alpha 1(X) collagen mRNAs

K Elima et al. Biochim Biophys Acta. .

Abstract

We have used polymerase chain reaction (PCR) technology and available cross-species sequence information to construct cDNA probes for mouse alpha 2(IX) and alpha 1(X) collagen transcripts. Sequencing confirmed the identification of the clones. Northern analysis proved sufficient divergence of the cloned sequences from other collagen transcripts: specific detection of the mouse 2.9 kb alpha 2(IX) and 3.3 kb alpha 1(X) collagen mRNAs was seen under normal hybridization and washing conditions.

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