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. 1992 Feb 20;1124(1):95-100.
doi: 10.1016/0005-2760(92)90131-e.

Two separate pools of sphingomyelin in BHK cells

P Quinn  1 , D Allan
Affiliations

Two separate pools of sphingomyelin in BHK cells

P Quinn et al. Biochim Biophys Acta. .

Abstract

In BHK cells labelled to equilibrium with [3H]choline and treated with sphingomyelinase the surface pool of sphingomyelin is degraded very rapidly (half-time 10 min) but the internal pool of sphingomyelin which accounts for about 30% of the total is only degraded slowly (half-time about 80 h) showing that the internal pool does not normally reach the surface. In [3H]choline incorporation experiments the internal pool begins to accumulate radioactivity at about the same time as phosphatidylcholine (30 min) but label does not enter the surface pool of sphingomyelin for a further 90 min. The internal and external pools reach the same specific activity only after about 20 h. Pulse-chase analysis with [3H]choline shows that radioactivity in each pool of sphingomyelin continues to increase when the specific radioactivity of phosphatidylcholine is decreasing, consistent with both pools being synthesised from a phosphatidylcholine precursor. The results suggest that sphingomyelin in BHK cells is present not only in the plasma membrane but also in a more rapidly labelling pool which does not mix with the surface pool.

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