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. 1978 Oct;75(10):5132-6.
doi: 10.1073/pnas.75.10.5132.

Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice

R M Steinman, M D Witmer

Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice

R M Steinman et al. Proc Natl Acad Sci U S A. 1978 Oct.

Abstract

Dendrite cells (DCs) are a new cell type initially identified in mouse lymphoid organs. Recently, DCs have been purified from mouse spleen. This paper demonstrates a functional role of DCs: they are potent stimulators of the primary mixed leukocytes reaction (MLR). As few as 300-1000 DCs doubled the proliferative activity of 5 X 10(6) allogeneic responder spleen cells, while 0.3-1.0 X 10(5) DCs induced a maximal stimulation of 30- to 80-fold. Between these extremes, the log of the MLR response increased linearly with the log of DC numbers. This dose-response assay was then used to compare the potency of purified DCs with that of other heterogeneous lymphoid populations, many of which gave dose-response curves with similar slopes. The potency of purified DCs as MLR stimulators was 100-300 times greater than that of unfractionated spleen cells. When spleen cells were fractionated by simple physical techniques, MLR-stimulating capacity in the subpopulations correlated closely with DC numbers. Removal of splenic B or T lymphocytes, by anti-immunoglobulin or anti-brain serum plus complement, did not reduce MLR-stimulating capacity. Finally, several populations, enriched in mononuclear phagocytes but lacking in DCs, stimulated weakly if at all. We conclude that DCs are a potent stimulating cell and are at least 100 times more effective than other major cell subclasses--i.e., B and T lymphocytes and macrophages.

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References

    1. J Exp Med. 1975 Jan 1;141(1):263-8 - PubMed
    1. J Exp Med. 1975 Jun 1;141(6):1390-9 - PubMed
    1. Immunol Rev. 1978;40:153-80 - PubMed
    1. J Exp Med. 1975 Apr 1;141(4):804-20 - PubMed
    1. J Immunol. 1975 May;114(5):1541-7 - PubMed

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