The cell wall subproteome of Listeria monocytogenes
- PMID: 15378750
- DOI: 10.1002/pmic.200400928
The cell wall subproteome of Listeria monocytogenes
Abstract
The surface subproteome of Listeria monocytogenes that includes many proteins already known to be involved in virulence and interaction with host cells has been characterized. A new method for the isolation of a defined surface proteome of low complexity has been established based on serial extraction of proteins by different salts at high concentration, and in all 55 proteins were identified by N-terminal sequencing and mass spectrometry. About 16% of these proteins are of unknown function and three proteins have no orthologue in the nonpathogenic L. innocua and might be involved in virulence mechanisms. Remarkably, a relatively high number of proteins with a function in the cytoplasmic compartment was identified in this surface proteome. These proteins had neither predicted or detectable signal peptides nor could any modification be observed except removal of the N-terminal methionine. Enolase (Lmo2455) is one of these proteins. It was shown to be present in the cell wall of the pathogen by immunoelectron microscopy and, along with heat shock factor DnaK (Lmo1473), elongation factor TU (Lmo2653), and glyceraldehyde-3-phosphate dehydrogenase (Lmo2459), it was found to be able to bind human plasminogen in overlay blots and surface plasmon resonance (SPR) experiments. The KD values of these interactions were determined by SPR measurements. The data indicate a possible role of these proteins as receptors for human plasminogen on the bacterial cell surface. The potential role of this recruitment of a host protease for extracellular invasion mechanisms is discussed.
Similar articles
-
Comparative proteome analysis of secretory proteins from pathogenic and nonpathogenic Listeria species.Proteomics. 2005 Apr;5(6):1544-57. doi: 10.1002/pmic.200401024. Proteomics. 2005. PMID: 15838904
-
Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen.Int J Biochem Cell Biol. 2007;39(6):1190-203. doi: 10.1016/j.biocel.2007.03.008. Epub 2007 Mar 15. Int J Biochem Cell Biol. 2007. PMID: 17449317
-
Insight into the core and variant exoproteomes of Listeria monocytogenes species by comparative subproteomic analysis.Proteomics. 2009 Jun;9(11):3136-55. doi: 10.1002/pmic.200800765. Proteomics. 2009. PMID: 19526548
-
Elucidation of mechanisms of acid stress in Listeria monocytogenes by proteomic analysis.Methods Biochem Anal. 2006;49:75-88. Methods Biochem Anal. 2006. PMID: 16929674 Review. No abstract available.
-
Protein N-terminal methionine excision.Cell Mol Life Sci. 2004 Jun;61(12):1455-74. doi: 10.1007/s00018-004-3466-8. Cell Mol Life Sci. 2004. PMID: 15197470 Free PMC article. Review.
Cited by
-
Non-classical protein secretion in bacteria.BMC Microbiol. 2005 Oct 7;5:58. doi: 10.1186/1471-2180-5-58. BMC Microbiol. 2005. PMID: 16212653 Free PMC article.
-
Protective properties and surface localization of Plasmodium falciparum enolase.Infect Immun. 2007 Nov;75(11):5500-8. doi: 10.1128/IAI.00551-07. Epub 2007 Sep 4. Infect Immun. 2007. PMID: 17785475 Free PMC article.
-
Interaction of Leptospira elongation factor Tu with plasminogen and complement factor H: a metabolic leptospiral protein with moonlighting activities.PLoS One. 2013 Nov 27;8(11):e81818. doi: 10.1371/journal.pone.0081818. eCollection 2013. PLoS One. 2013. PMID: 24312361 Free PMC article.
-
A homolog of glyceraldehyde-3-phosphate dehydrogenase from Riemerella anatipestifer is an extracellular protein and exhibits biological activity.J Zhejiang Univ Sci B. 2014 Sep;15(9):776-87. doi: 10.1631/jzus.B1400023. J Zhejiang Univ Sci B. 2014. PMID: 25183032 Free PMC article.
-
Protein as chemical cue: non-nutritional growth enhancement by exogenous protein in Pseudomonas putida KT2440.PLoS One. 2014 Aug 12;9(8):e103730. doi: 10.1371/journal.pone.0103730. eCollection 2014. PLoS One. 2014. PMID: 25117434 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
