GMAP-210 recruits gamma-tubulin complexes to cis-Golgi membranes and is required for Golgi ribbon formation
- PMID: 15294158
- DOI: 10.1016/j.cell.2004.07.012
GMAP-210 recruits gamma-tubulin complexes to cis-Golgi membranes and is required for Golgi ribbon formation
Abstract
Mammalian cells concentrate Golgi membranes around the centrosome in a microtubule-dependent manner. The mechanisms involved in generating a single Golgi ribbon in the periphery of the centrosome remain unknown. Here we show that GMAP-210, a cis-Golgi microtubule binding protein, recruits gamma-tubulin-containing complexes to Golgi membranes even in conditions where microtubule polymerization is prevented and independently of Golgi apparatus localization within the cell. Under overexpression conditions, very short microtubules, or tubulin oligomers, are stabilized on Golgi membranes. GMAP-210 depletion by RNA interference results in extensive fragmentation of the Golgi apparatus, supporting a role for GMAP-210 in Golgi ribbon formation. Targeting of GMAP-210 or its C terminus to mitochondria induces the recruitment of gamma-tubulin to their surface and redistribution of mitochondria to a pericentrosomal location. All our experiments suggest that GMAP-210 displays microtubule anchoring and membrane fusion activities, thus contributing to the assembly and maintenance of the Golgi ribbon around the centrosome.
Comment in
-
Positioning the Golgi apparatus.Cell. 2004 Aug 6;118(3):271-2. doi: 10.1016/j.cell.2004.07.015. Cell. 2004. PMID: 15294150
Similar articles
-
The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi.J Cell Biol. 2004 Oct 25;167(2):281-92. doi: 10.1083/jcb.200407088. J Cell Biol. 2004. PMID: 15504911 Free PMC article.
-
GMAP-210, A cis-Golgi network-associated protein, is a minus end microtubule-binding protein.J Cell Biol. 1999 Apr 5;145(1):83-98. doi: 10.1083/jcb.145.1.83. J Cell Biol. 1999. PMID: 10189370 Free PMC article.
-
Positioning the Golgi apparatus.Cell. 2004 Aug 6;118(3):271-2. doi: 10.1016/j.cell.2004.07.015. Cell. 2004. PMID: 15294150
-
Golgi positioning: are we looking at the right MAP?J Cell Biol. 2005 Mar 28;168(7):993-8. doi: 10.1083/jcb.200501088. Epub 2005 Mar 21. J Cell Biol. 2005. PMID: 15781478 Free PMC article. Review.
-
Regulation of microtubule nucleation mediated by γ-tubulin complexes.Protoplasma. 2017 May;254(3):1187-1199. doi: 10.1007/s00709-016-1070-z. Epub 2017 Jan 10. Protoplasma. 2017. PMID: 28074286 Review.
Cited by
-
Cell cycle regulation of Golgi membrane dynamics.Trends Cell Biol. 2013 Jun;23(6):296-304. doi: 10.1016/j.tcb.2013.01.008. Epub 2013 Feb 28. Trends Cell Biol. 2013. PMID: 23453991 Free PMC article. Review.
-
The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi.J Cell Biol. 2004 Oct 25;167(2):281-92. doi: 10.1083/jcb.200407088. J Cell Biol. 2004. PMID: 15504911 Free PMC article.
-
Conserved motif of CDK5RAP2 mediates its localization to centrosomes and the Golgi complex.J Biol Chem. 2010 Jul 16;285(29):22658-65. doi: 10.1074/jbc.M110.105965. Epub 2010 May 13. J Biol Chem. 2010. PMID: 20466722 Free PMC article.
-
Golgi Feels Its Own Wound.Adv Wound Care (New Rochelle). 2013 Apr;2(3):87-92. doi: 10.1089/wound.2011.0352. Adv Wound Care (New Rochelle). 2013. PMID: 24527331 Free PMC article. Review.
-
Regulation of primary cilia formation by ceramide.J Lipid Res. 2009 Oct;50(10):2103-10. doi: 10.1194/jlr.M900097-JLR200. Epub 2009 Apr 16. J Lipid Res. 2009. PMID: 19372594 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
