The life cycle of SARS coronavirus in Vero E6 cells

doi:10.1002/jmv.20095

. 2004 Jul;73(3):332-7.

doi: 10.1002/jmv.20095.

The life cycle of SARS coronavirus in Vero E6 cells

Zhang Qinfen¹, Cui Jinming, Huang Xiaojun, Zheng Huanying, Huang Jicheng, Fang Ling, Li Kunpeng, Zhang Jingqiang

Affiliations

PMID: 15170625
PMCID: PMC7166737
DOI: 10.1002/jmv.20095

The life cycle of SARS coronavirus in Vero E6 cells

Zhang Qinfen et al. J Med Virol. 2004 Jul.

. 2004 Jul;73(3):332-7.

doi: 10.1002/jmv.20095.

Authors

Zhang Qinfen¹, Cui Jinming, Huang Xiaojun, Zheng Huanying, Huang Jicheng, Fang Ling, Li Kunpeng, Zhang Jingqiang

Affiliation

¹ State Key Lab for Biocontrol, Zhongshan University, Guangzhou, China.

PMID: 15170625
PMCID: PMC7166737
DOI: 10.1002/jmv.20095

Abstract

The aim of the study was to establish the life cycle of severe acute respiratory syndrome-associated coronavirus (SARS CoV) in host cells and determine the pathogenesis of SARS. Vero E6 cells (African green monkey kidney cells) were inoculated with SARS coronavirus for 3, 7, 24, 48, and 72 hr, respectively, and were observed under electron microscope. It was found that the SARS coronavirus entered the cells through membrane fusion instead of endocytosis, and then the nucleocapsids assembled in the RER and matured by budding into the smooth vesicles, which were derived from the Golgi apparatus. The smooth vesicles fused with the cell membrane, and the mature particles were released. A special phenomenon was that some virus-like particles appeared in the nucleus. We propose a scheme of the life cycle of SARS coronavirus and discuss the mechanism of its replication in Vero E6 cells.

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[1] Beesley JE, Hitchcock LM. 1982. The ulturastructure of Feline infectious peritonitis virus in feline embryonic lung cells. J Gen Virol 59: 23–28. - PubMed

[2] Beesley JE, Hitchcock LM. 1982. The ulturastructure of Feline infectious peritonitis virus in feline embryonic lung cells. J Gen Virol 59: 23–28. - PubMed

[3] Chen H, Wurm T, Britton P, Brooks G. 2002. Interaction of coronavirus nucleoprotein wieh nucleolar antigens and the host cell. J Virol 76: 5233–5250. - PMC - PubMed

[4] Chen H, Wurm T, Britton P, Brooks G. 2002. Interaction of coronavirus nucleoprotein wieh nucleolar antigens and the host cell. J Virol 76: 5233–5250. - PMC - PubMed

[5] Collins AR. 1993. HLA class I antigen serves as a receptor for human coronavirus OC43. Immunol Invest 22: 95–103. - PubMed

[6] Collins AR. 1993. HLA class I antigen serves as a receptor for human coronavirus OC43. Immunol Invest 22: 95–103. - PubMed

[7] Drosten C, Gunther S, Preiser W, van der Werf S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguiere AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Muller S, Rickerts V, Sturmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348: 1967–1976. - PubMed

[8] Drosten C, Gunther S, Preiser W, van der Werf S, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguiere AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Muller S, Rickerts V, Sturmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348: 1967–1976. - PubMed

[9] Ducatelle R, Coussement W, Pensaert MB, Debouck P, Hoorens J. 1981. In vivo morphogenesis of a new porcine enteric coronavirus, CV 777. Arch Virol 68: 35–44. - PMC - PubMed

[10] Ducatelle R, Coussement W, Pensaert MB, Debouck P, Hoorens J. 1981. In vivo morphogenesis of a new porcine enteric coronavirus, CV 777. Arch Virol 68: 35–44. - PMC - PubMed

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The life cycle of SARS coronavirus in Vero E6 cells

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The life cycle of SARS coronavirus in Vero E6 cells

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