Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes
- PMID: 15167971
- DOI: 10.1007/s00011-004-1247-3
Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes
Abstract
Objective: Histamine stimulates nerve growth factor (NGF) secretion from cultured astrocytes. Histamine H(1)-receptor antagonists completely block its effect. In the present study, we determined the involvement of histamine-receptor subtypes in this process.
Materials and methods: Radioligand-binding assay was used to establish the presence of histamine H(1)- and H(2)-receptors on new-born rat cortical astrocytes in primary culture. Histamine H(1)-, H(2)- and H(3)/H(4)-receptor ligands, and highly selective protein kinase C (PKC) inhibitor were used to influence NGF secretion from cultured astrocytes. NGF, released into the culture medium, was measured by NGF-ELISA.
Results: Histamine H(1)-receptor agonists (histamine, selected histaprodifens) increased the secretion of NGF from cultured astrocytes in a concentration-dependent manner. H(1)-receptor antagonists/inverse agonists (mepyramine, triprolidine) and PKC inhibitor completely blocked the effect of histamine. Histamine H(2)- and H(3)-receptor agonists did not enhance NGF secretion significantly. In addition, H(2)- and H(3)/H(4)-receptor antagonists did not diminish histamine-stimulated NGF release.
Conclusions: Our results indicate that histamine H(1)-receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.
Similar articles
-
The synergistic effect of histamine and IL-6 on NGF secretion from cultured astrocytes is evoked by histamine stimulation of IL-6 secretion via H1-receptor-PKC-MAPK signalling pathway.Inflamm Res. 2008;57 Suppl 1:S33-4. doi: 10.1007/s00011-007-0617-z. Inflamm Res. 2008. PMID: 18345501 No abstract available.
-
Multiple role of histamine H1-receptor-PKC-MAPK signalling pathway in histamine-stimulated nerve growth factor synthesis and secretion.Biochem Pharmacol. 2006 Nov 30;72(11):1375-81. doi: 10.1016/j.bcp.2006.06.028. Epub 2006 Aug 1. Biochem Pharmacol. 2006. PMID: 16884695 Review.
-
Involvement of histaminergic receptor mechanisms in the stimulation of NT-3 synthesis in astrocytes.Neuropharmacology. 2011 Jun;60(7-8):1309-17. doi: 10.1016/j.neuropharm.2011.01.019. Epub 2011 Jan 27. Neuropharmacology. 2011. PMID: 21276809
-
The influence of histamine H1-, H2- and H3-receptor antagonists on histamine stimulated NGF release from cultured astrocytes.Inflamm Res. 2001 Apr;50 Suppl 2:S84-5. doi: 10.1007/PL00022418. Inflamm Res. 2001. PMID: 11411616 No abstract available.
-
Delineation of receptor-ligand interactions at the human histamine H1 receptor by a combined approach of site-directed mutagenesis and computational techniques - or - how to bind the H1 receptor.Arch Pharm (Weinheim). 2005 Jun;338(5-6):248-59. doi: 10.1002/ardp.200400998. Arch Pharm (Weinheim). 2005. PMID: 15952243 Review.
Cited by
-
Trafficking and fusion of neuropeptide Y-containing dense-core granules in astrocytes.J Neurosci. 2008 Dec 17;28(51):13815-27. doi: 10.1523/JNEUROSCI.5361-07.2008. J Neurosci. 2008. PMID: 19091972 Free PMC article.
-
Physiology of Astroglia.Physiol Rev. 2018 Jan 1;98(1):239-389. doi: 10.1152/physrev.00042.2016. Physiol Rev. 2018. PMID: 29351512 Free PMC article. Review.
-
Histamine Induces Alzheimer's Disease-Like Blood Brain Barrier Breach and Local Cellular Responses in Mouse Brain Organotypic Cultures.Biomed Res Int. 2015;2015:937148. doi: 10.1155/2015/937148. Epub 2015 Nov 30. Biomed Res Int. 2015. PMID: 26697497 Free PMC article.
-
NGF and Its Receptors in the Regulation of Inflammatory Response.Int J Mol Sci. 2017 May 11;18(5):1028. doi: 10.3390/ijms18051028. Int J Mol Sci. 2017. PMID: 28492466 Free PMC article. Review.
-
Inhibition of apoptosis by P2Y2 receptor activation: novel pathways for neuronal survival.J Neurosci. 2006 Apr 5;26(14):3798-804. doi: 10.1523/JNEUROSCI.5338-05.2006. J Neurosci. 2006. PMID: 16597733 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
