doi: 10.1038/nsmb777.
Epub 2004 May 23.
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain
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- PMID: 15156196
- DOI: 10.1038/nsmb777
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Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain
Nat Struct Mol Biol.
2004 Jun.
Abstract
We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.
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