The use of scanning ion conductance microscopy to image A6 cells
- PMID: 15134807
- DOI: 10.1016/j.mce.2003.10.015
The use of scanning ion conductance microscopy to image A6 cells
Abstract
Background: Continuous high spatial resolution observations of living A6 cells would greatly aid the elucidation of the relationship between structure and function and facilitate the study of major physiological processes such as the mechanism of action of aldosterone. Unfortunately, observing the micro-structural and functional changes in the membrane of living cells is still a formidable challenge for a microscopist.
Method: Scanning ion conductance microscopy (SICM), which uses a glass nanopipette as a sensitive probe, has been shown to be suitable for imaging non-conducting surfaces bathed in electrolytes. A specialized version of this microscopy has been developed by our group and has been applied to image live cells at high-resolution for the first time. This method can also be used in conjunction with patch clamping to study both anatomy and function and identify ion channels in single cells.
Results: This new microscopy provides high-resolution images of living renal cells which are comparable with those obtained by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Continuous 24h observations under normal physiological conditions showed how A6 kidney epithelial cells changed their height, volume, and reshaped their borders. The changes in cell area correlated with the density of microvilli on the surface. Surface microvilli density ranged from 0.5 microm(-2) for extended cells to 2.5 microm(2) for shrunk cells. Patch clamping of individual cells enabled anatomy and function to be correlated.
Conclusions: Scanning ion conductance microscopy provides unique information about living cells that helps to understand cellular function. It has the potential to become a powerful tool for research on living renal cells.
Similar articles
-
[High-resolution patch-clamp technique based on feedback control of scanning ion conductance microscopy].Sheng Li Xue Bao. 2010 Jun 25;62(3):275-83. Sheng Li Xue Bao. 2010. PMID: 20571746 Chinese.
-
Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system.Biophys J. 2002 Dec;83(6):3296-303. doi: 10.1016/S0006-3495(02)75330-7. Biophys J. 2002. PMID: 12496097 Free PMC article.
-
Angular Approach Scanning Ion Conductance Microscopy.Biophys J. 2016 May 24;110(10):2252-65. doi: 10.1016/j.bpj.2016.04.017. Biophys J. 2016. PMID: 27224490 Free PMC article.
-
The scanning ion conductance microscope for cellular physiology.Am J Physiol Heart Circ Physiol. 2013 Jan 1;304(1):H1-11. doi: 10.1152/ajpheart.00499.2012. Epub 2012 Oct 19. Am J Physiol Heart Circ Physiol. 2013. PMID: 23086993 Review.
-
Scanning ion conductance microscopy for studying biological samples.Sensors (Basel). 2012 Nov 6;12(11):14983-5008. doi: 10.3390/s121114983. Sensors (Basel). 2012. PMID: 23202197 Free PMC article. Review.
Cited by
-
Operando Scanning Electrochemical Probe Microscopy during Electrocatalysis.Chem Rev. 2023 Apr 26;123(8):4972-5019. doi: 10.1021/acs.chemrev.2c00766. Epub 2023 Mar 27. Chem Rev. 2023. PMID: 36972701 Free PMC article. Review.
-
Scanning Ion Conductance Microscopy.Chem Rev. 2021 Oct 13;121(19):11726-11768. doi: 10.1021/acs.chemrev.0c00962. Epub 2020 Dec 9. Chem Rev. 2021. PMID: 33295182 Free PMC article. Review.
-
Anti-inflammatory effect of Lycium barbarum on polarized human intestinal epithelial cells.Nutr Res Pract. 2019 Apr;13(2):95-104. doi: 10.4162/nrp.2019.13.2.95. Epub 2019 Mar 11. Nutr Res Pract. 2019. PMID: 30984353 Free PMC article.
-
Nanopipettes as Monitoring Probes for the Single Living Cell: State of the Art and Future Directions in Molecular Biology.Cells. 2018 Jun 6;7(6):55. doi: 10.3390/cells7060055. Cells. 2018. PMID: 29882813 Free PMC article. Review.
-
Multifunctional scanning ion conductance microscopy.Proc Math Phys Eng Sci. 2017 Apr;473(2200):20160889. doi: 10.1098/rspa.2016.0889. Epub 2017 Apr 12. Proc Math Phys Eng Sci. 2017. PMID: 28484332 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous