Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1
- PMID: 1511901
- DOI: 10.1016/0378-1119(92)90200-9
Cloning and sequence of a functionally active cDNA encoding the mouse ubiquitin-activating enzyme E1
Abstract
A cDNA encoding the ubiquitin-activating enzyme, E1, was isolated from the mouse mammary carcinoma cell line, FM3A, and shown to complement mutant mouse cells deficient in the enzyme. The 3495-bp cDNA encodes 1058 amino acids (aa), and shares extensive homology with the human E1 enzyme at both the nucleotide and aa sequence levels.
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